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Questions related from Evgeny V. Denisov
We isolate the RNA from tumor cells which were obtained by laser microdissection, using RNeasy Micro Plus Kit (Qiagen). The concentration of the RNA varies from 7 to 9 ng/ul, RIN - 5.0-8.8,...
03 March 2014 7,373 12 View
I am going to perform whole genome expression profiling of different tumor cells of one breast tumor. I isolate these tumor cells by laser microdissection and extract RNA. The total RNA...
10 October 2013 3,966 12 View
I have a problem with preparation of sections from frozen samples of normal breast tissue containing abundant fat tissue. These samples are not frozen in cryotome (from -20 to -30C). So, I can not...
05 May 2013 2,829 29 View
Does anybody have experience in whole transcriptome amplification from cells? My usual study includes RNA extraction from cells (n=30-300), isolated from frozen tumors by laser-capture...
11 November 2012 3,884 4 View
I am looking for data about the expression of homeobox genes, namely HOXC6, in tumor spheroids including spheres formed by cancer stem cells and in architectural patterns of tumor cells in breast...
11 November 2012 9,195 0 View
I have some troubles with understanding terms "cancer stem cells" and "non-cycling (resting) cells". For example, somebody write that core of tumorspheres (spheroids) contains cancer stem cells,...
11 November 2012 1,202 9 View
Do you have any experience in in situ PCR? I think it is good approach to study intratumoral heterogeneity but I do not know nothing about it. In particular, where can I find thermal cycler for in...
10 October 2012 6,349 4 View
If you have information demonstrating influence of chemotherapy on miRNA level, please share with me links. Thank you in advance!
09 September 2012 9,237 2 View
