Do you have any experience in in situ PCR? I think it is good approach to study intratumoral heterogeneity but I do not know nothing about it. In particular, where can I find thermal cycler for in situ PCR? All PCR machines I have found are sold as second hand. So, does it mean that in situ PCR is outdated method? Why this approach didn't gain popularity in molecular pathology of cancer?
If you are looking for fixed mammalian cells, best way is Olink biosciences(http://www.olink.com/) method called PLA (Proximity ligation assay). Though it would not answer you completely for situ PCR alone as the assay focus is to find your two interested proteins are proximal enough/interacting.
An interesting alternative could be considered!
http://www.techne.com/product.asp?dsl=4060
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