I want to show RNA delivery between cells using stained RNA.
Mostly used is pyronin Y. Look at http://onlinelibrary.wiley.com/doi/10.1002/cyto.990080206/pdf or http://www.flowcyt.rutgers.edu/main%20pages/Protocols/DNA%20and%20RNA%20Quantitation%20using%20Pyronin%20Y%20and%20Hoechst%2033342.doc
The Molecular Probes handbook continues to be a useful reference for fluorophore information.
Have a look at the following link (I used tinyurl converter since it's a long string otherwise).
http://tinyurl.com/nzb25ex
SYTO RNASelect Green-Fluorescent Cell Stain should suit your need, and there are other choices also described on that page.
Thanks!
looks good, I try it
My research includes monitoring canopy temperature over time with a thermal FLIR camera. I am looking for a code/program that will help me analyze the videos easily and automatically. For my...
24 February 2020 4,722 3 View
Hi, I've been using growthcurver package for growth curves experiment. I have the output data per well- but now I want to group wells together according to my meta-data (for instance three...
21 June 2019 6,635 1 View
Hi all, I try to figure out weather there is a change in my rRNA following treatment. when I treat the cells (mouse cells) and isolated RNA, I see a major difference in RNA concentration, without...
06 September 2018 724 7 View
Hello, I'm trying to amplify a gene in both eDNA and bacterial DNA (a strain of Bacillus, that should be the positive control) using degenerative primers, their sequence was published in a...
10 December 2017 503 3 View
I am trying to identify by flow cytometry osteogenic and chondrogenic lineages differentiated from MSCs in human BM. What would be the best markers (intracellular or extracellular) for this...
27 September 2017 7,371 3 View
24 July 2016 5,940 3 View
I am on the lookout for the Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence. Does anyone know where I can find it? Thank you in advance
03 March 2021 3,568 1 View
Gel electrophoresis, RNA degradation, RNA extraction from fresh tissue
02 March 2021 5,433 5 View
I am going to have 3 different probes in my qPCR work that I am going to do. But I realized that the machine we have in the lab is a Rotor-Gene Q 2plex HRM Platform, saying it has green, yellow,...
01 March 2021 8,544 1 View
We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...
01 March 2021 3,355 3 View
I transfected my LNCaP-WT cells with 3 shRNA plus their NTC two weeks ago and split two puromycin selected cell plates on Friday last week(Feb 26). I checked for GFP in the cells, and they all...
28 February 2021 4,949 3 View
Interested to stain mice brain tissue with WGA 488 conjugate, appreciate if anyone willing to offer me some guidance or suggestions! Thank you!
28 February 2021 3,951 2 View
I have two groups of brain samples, control and treated for example. It was total RNA nova seq sequencing. I tried all the available pipeline like: star+rsem+deseq2, Hista+stringtie+cuffdiff,...
27 February 2021 356 6 View
Hi everybody, I have been running a multiplex IHC which allow me to stain the same tissue with different markers (Primary Ab + Secondary conjugated Ab) (AF487, AF555, AF647). Since I would like to...
27 February 2021 5,028 2 View
Hello, I am looking for some expertise on culturing MCF7s. I purchased them from ATCC and I have been following all media and culture conditions. Media is EMEM with 10% FBS and 10ug/mL of...
27 February 2021 3,904 2 View
Hello, I have been struggling with the electroporation of PBMC with HIV-I mRNA. Although the quality of the RNA generated is good, and the handling of the cells in meticulous, the frequency of...
27 February 2021 9,233 3 View