I need a colon cancer cell line where there is no mutation present in p53 for cytotoxic studies.
One of the most popular cell line in this case will be HCT116 +/+. It also has an advantage of having artificially made counterpart, namely, HCT116 -/- that is wt p53 null (though, in fact it expresses detectable levels of truncated p53 protein). Both cell lines are available from a number of researchers but were originally used by Bert Vogelstein group.
According to the cBioPortal, the following colon cancer lines are p53 wild type:
SW480, SW48, SW620, SW 948, COLO205, CW2, HRT18, HS675T, HS698T, HT29, and HT55. There may be more, or course.
Thanks. I have worked with Ht-29 and Caco.2 cell lines, and both have mutated for APC and p53 genes. Now I wanted to include a non mutated p53 cell line. I will take in consideration your differents options.
Regarding HT29, The Broad Institute's Cancer Cell line Encyclopedia (http://www.broadinstitute.org/software/cprg/?q=node/11; you must register to search but its free) does not list the TP53 mutation to which Oleg refers (R273H). The Broad resource is reportedly based on their own sequencing, not prior literature. Perhaps there are different HT29 lines out there. I just checked the Sanger cell line project as Rohan suggested and indeed, HT29 is listed as having the R273H mutation in agreement with Oleg (http://cancer.sanger.ac.uk/cell_lines/sample/overview?id=905939). So the two databases, each performing their own verification, disagree regarding TP53 in HT29.
To update my original answer, after checking both the Broad and Sanger databases, there are several discrepancies, but both list SW48 as TP53 wild type, and CW2 as having the silent mutation P47P.
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