Could anyone please suggest me how to do methanol calibration in (%) level using online gas chromotography?
Anyone tried adapting UMNSAH/DF-1 (Chicken Fibroblast cells) from adherent to suspension cells. Currently we are using Xerumfree TnC bio serum alternative with DMEM. The cells are not adapting...
05 May 2024 3,316 1 View
Do they have any protein coding function, or they have other specific roles? Please help me understand this. Thank you in advance....
24 March 2024 259 0 View
I'm encountering a persistent problem with cell count stagnation in my fed- batch process with CHO S cell line. Initially, after seeding on day 5-6, the cell count reaches 6-8 million cells/ml,...
20 March 2024 8,611 6 View
It's been more than 20 days they are not getting attached to surface of flask.
04 March 2024 8,594 0 View
At first try, I got the cell but after that I'm not getting the cell. Can someone help me where I must be going wrong during the procedure.
04 March 2024 3,408 1 View
I have a protein who's structure has disordered regions. so i modelled the disordered regions using I-TASSER. Since the protein is too big I had to model them in domains and I even tried I-TASSER...
25 February 2024 3,810 1 View
Hi, I have established a bioreactor parameters mammalian cell process with the following parameters: Setpoint Deadband PID settings 1) pH-...
14 December 2023 7,651 1 View
Hello ! I have recently concluded the data analysis of a study which looks at Attitude, Anxiety towards robots and Trust as predictors of intention to use robots. The Multiple Regression Analysis...
17 November 2023 5,722 3 View
In my experiment, I utilized a combination of Poloxamer 188 (Kolliphor) and Gibco Anticlumping agent in Dynamis media at a concentration of 0.1% per liter for CHO S cell culture. Surprisingly, I...
07 June 2023 7,493 1 View
What is the reason for cell clumping in the CHO-S cell line despite the presence of an anticlumping agent (1%/L) in Dynamis medium, when the cell density reaches above 3x10^6 cells/mL? Is the...
03 May 2023 7,484 3 View
Hello experts, Does anyone know any free software about retention index prediction ?
08 August 2024 7,403 2 View
Dear QE-users, In the method where full MS positive mode and PRM mode are used, we always get an incorrect auxiliary gas reading (41 instead of 25). This only happens in this method; other...
06 August 2024 4,953 0 View
Hello, Can anyone provide me with the absorption coefficient of methane gas at 7.7 um? Any reference?
06 August 2024 980 5 View
There are anaerobic gas pak jars and environment providers in the laboratory that provide anaerobic environmental conditions for the growth of anaerobic bacteria. However, there is no anaerobic...
05 August 2024 4,945 3 View
or we can collaborate
01 August 2024 6,705 2 View
I am working on algal extract to which gas chromatography (Not GC-MS) spectrum I want to discover. My question is can we identify specific compounds using retention time if I compared the RT with...
29 July 2024 8,034 4 View
List down the various factors responsible and how does this effect the overall biogas production when it is related to Hydraulic retention time of the digester based on the feedstock?
27 July 2024 4,800 4 View
Kindly share a research paper that explains the effect of Methanol and ethanol treatment on collagen based scaffolds. How it increases porosity and swelling. Kindly help me find a paper. Thank you
24 July 2024 3,937 0 View
Why methanol and sulphuric acid, used in the analysis of polyhydroxyalkanoates (PHA) methyl esters by GC-MS? Additionally, why do we typically use non-polar solvents in GC-MS?
22 July 2024 1,210 2 View
im studing gaussian 16 with reading paper about I-131 Metaiodobenzylguanidine in the paper "In a similar vein, nuclear magnetic resonance shielding values were investigated using the widely...
16 July 2024 6,040 4 View