What is the reason for cell clumping in the CHO-S cell line despite the presence of an anticlumping agent (1%/L) in Dynamis medium, when the cell density reaches above 3x10^6 cells/mL? Is the observed clumping specific to the CHO-S cell strain being cultured or a general issue with CHO cells in suspension culture? Additionally, what alternatives are available to reduce the cost of procuring an anticlumping agent, such as identifying less expensive chemicals that exhibit similar anti-clumping properties as pluronic and poloxamer?
Hi Ashwin Naidu
The cell we use does not clump at density of 3 millions in 1 ml.
Good choices DNAase, EDTA, and make sure the centrifugation time and RPM are optimized well .
Hello! Clumping might happen due to contamination of the cell lines. However, if there is no sign of contamination, clumping might occur due to other stresses affecting the cell line, like mentioned before, centrifugation times and rpm might be the cause as mechanical force can provoke clumping.
Another thing you should investigate if there is any nutrient deficiency in the culture medium. Have you check the other culture medias as well ?
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