09 February 2017 1 1K Report

I am culturing cortical neurons from E15.5 mice on PDL and laminin coated plates. I plate them in Neurobasal medium supplemented with FBS, 0.6 mM L-Glut and Pen-strep. After 24 hr, I switch them to maintenance medium: Neurobasal medium with B27 supplement and pen/strep. Typically the cells in culture stay good for ~ 7 days but start dying after that. I wonder if it's because of the lack of L-Glutamine in the medium. But we have followed this protocol in a lab for a long time. Any suggestion/information on whether or not to have L-Glut and the reason behind would help.

Thanks!

More Pavithra Aravamudhan's questions See All
One way ANOVA followed by Dunnett's post hoc test?

I am doing a research in biotechnology where I had my animal studies results. The SD values of the body weight of the animals were conducted. I need to find out the statistical significant values...

23 September 2020 8,722 8 View

One way ANOVA followed by Dunnett's post hoc test?

23 September 2020 5,978 3 View

Reduction in islets size due to Focal cytoplasmic vacuolation?

When the rats were induced diabetes with the administration of Streptozotocin Section from pancreatic islets and acini shows cytoplasmic vacuolation. Islets are small in size with reduced in the...

09 September 2020 9,282 2 View

How to Integrate of Sumo and Veins?

Hi everyone, I have installed omnet 4.3, sumo 0.21.0 and veins 3.0 (This version was necessary as I want to work with NETA framework) . I was able to run the example from Veins and also I...

07 January 2020 2,824 1 View

Psychometric tests and student profiling/competency mapping

Hi all, I am designing a student profiling system (in university level technical education) in which student competencies are mapped and used for further intervention. I want to test achievement...

31 May 2017 1,552 11 View

How to do microbial extraction for isolation and identification of secondary metabolites?

20 April 2017 6,010 4 View

Will overloading an inductor in buck converter affect its performance?

When we increase the load current in buck converter, capacitance and inductance values needs to be over dimensioned. What happens to its characteristics if the same value of inductor is retained?...

03 November 2016 440 7 View

Do you always do a no RT control for every RNA sample that has been independently extracted and from which cDNA has been prepared cDNA?

We are just starting to do real time RT-PCR and I have question about controls for real time RT-PCR. Do you always do a no RT control for every RNA sample that has been independently extracted and...

20 September 2016 2,535 2 View

Why is CTAB is more preferred in isolating DNA from plant sources?

I need a comparison with SDS.

25 December 2013 1,334 12 View

What is the minimum yield of phycocyanin extracted from blue green algae?

The extract is dissolved in the buffer and I haven't dried the compound.

16 December 2013 1,696 1 View

Similar questions and discussions
Can we classify HV and LV lines based on line length?

I have dataset which shows the length of power lines. I need to classify the lines based on the line length. Is there a rule to classify the High voltage (HV) and low voltage (LV) lines based on...

03 March 2021 4,116 4 View

No title

02 March 2021 3,060 3 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

Do you need the CMV enhancer before the CMV promotor for a proper function of CMV on the gene that follows?

I'm a student and I have to produce a cell line with a knockin for the NRF2 gene with GFP. I have to put a promotor in front of the GFP because the gene will be too far away from the promotor of...

28 February 2021 7,127 2 View

What could be some reasons why unstimulated monocytes are getting activated?

Im doing PBMC isolation -> CD14+ enrichment using magnetic beads -> stimulation setup. My negative control is just cells in cRPMI but they seem to get activated over and over again.

28 February 2021 7,883 3 View

No title

28 February 2021 9,936 2 View

When is it unnecessary to change organisational culture?

As a former management practitioner, I recall a generally accepted view that changing organisational culture is difficult, so you should only attempt it if the organisational change that you are...

27 February 2021 2,595 8 View

Our partners will collect data and use cookies for ad personalization and measurement. Learn how we and our ad partner Google, collect and use data. Agree & close