I wanted to quantify the amount of silver released from the chitosan scaffolds. I am getting a gradience of results through AAS. The total release of Ag from the scaffolds experimentally is not matching with the theoretical value present in the scaffold. How do i troubleshoot this problem?
Hi, there are two ways you can determine the presence of any metal substance in a scaffolds.
1. You can do Inductively coupled plasma - optical emission spectrometry, (ICP-OES) or ICP-MS after incubating the samples in specific biological medium for a certain period of time (for leaching study) or after complete degradation.
2. You can also try with EDX-SEM analysis, this also gives you the approximate quantitative results about the presence. Additionally, the distribution of metals inside the scaffold. But this technique has some limitations too, you can try with multiple areas of a same sample to get the average quantitative results.