Pavithra Aravamudhan

5 Questions 11 Answers 0 Followers

Questions related from Pavithra Aravamudhan

Cortical neuron culture medium - whether or not to include L-Glutamine?

I am culturing cortical neurons from E15.5 mice on PDL and laminin coated plates. I plate them in Neurobasal medium supplemented with FBS, 0.6 mM L-Glut and Pen-strep. After 24 hr, I switch them...

09 February 2017 1,053 1 View

Neuron maturity and synapse formation in primary cultures?

I relatively new to working with primary neuronal cultures and have two basic questions. (1) What does neuronal maturity mean, in case of cortical or DRG neurons cultured from embryonic mice? (2)...

04 January 2017 6,550 5 View

Why would Ciliobrevin D not inhibit Dynein in neuronal cultures?

I have been trying to get Ciliobrevin D to inhibit dynein motor activity in primary neuronal (cortical and dorsal root ganglion) cultures. I am tracking axonal transport of lysosome as a proxy for...

27 August 2016 9,825 2 View

How do I get mouse DRG neurons to stick to the main channel in microfluidic devices?

I have been having difficulties in trying to culture mouse DRG neurons in microfluidic devices, that help isolate neuronal cell body from axons (bought from Xona SND150). My problem is with...

03 August 2016 5,918 5 View

Why would cerebellar tissue after papain digestion and trituration remain as a web rather than single-cell suspension?

I'm following the method from "http://www.jneurosci.org/content/18/10/3563.full.pdf" to isolate and culture cerebellar neurons (primarily interested in Purkinje). However, I am having trouble...

29 February 2016 2,961 8 View