Pavithra Aravamudhan

3 Questions 6 Answers 0 Followers

Questions related from Pavithra Aravamudhan

Cortical neuron culture medium - whether or not to include L-Glutamine?

I am culturing cortical neurons from E15.5 mice on PDL and laminin coated plates. I plate them in Neurobasal medium supplemented with FBS, 0.6 mM L-Glut and Pen-strep. After 24 hr, I switch them...

09 February 2017 1,047 1 View

How do I get mouse DRG neurons to stick to the main channel in microfluidic devices?

I have been having difficulties in trying to culture mouse DRG neurons in microfluidic devices, that help isolate neuronal cell body from axons (bought from Xona SND150). My problem is with...

03 August 2016 5,912 5 View

Why would cerebellar tissue after papain digestion and trituration remain as a web rather than single-cell suspension?

I'm following the method from "http://www.jneurosci.org/content/18/10/3563.full.pdf" to isolate and culture cerebellar neurons (primarily interested in Purkinje). However, I am having trouble...

29 February 2016 2,941 8 View