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Questions related from Pavithra Aravamudhan
I am culturing cortical neurons from E15.5 mice on PDL and laminin coated plates. I plate them in Neurobasal medium supplemented with FBS, 0.6 mM L-Glut and Pen-strep. After 24 hr, I switch them...
09 February 2017 1,053 1 View
I relatively new to working with primary neuronal cultures and have two basic questions. (1) What does neuronal maturity mean, in case of cortical or DRG neurons cultured from embryonic mice? (2)...
04 January 2017 6,550 5 View
I have been trying to get Ciliobrevin D to inhibit dynein motor activity in primary neuronal (cortical and dorsal root ganglion) cultures. I am tracking axonal transport of lysosome as a proxy for...
27 August 2016 9,825 2 View
I have been having difficulties in trying to culture mouse DRG neurons in microfluidic devices, that help isolate neuronal cell body from axons (bought from Xona SND150). My problem is with...
03 August 2016 5,918 5 View
I'm following the method from "http://www.jneurosci.org/content/18/10/3563.full.pdf" to isolate and culture cerebellar neurons (primarily interested in Purkinje). However, I am having trouble...
29 February 2016 2,961 8 View