27 September 2016 2 5K Report

Hi all,

So I've been working up a lab protocol for extracting DNA from oral and nasal swabs for 16S sequencing. Just when I thought I'd made my decision on swabs to use etc I get a recommendation to try the eNAT swabs from Copan! Has anyone used these for 16S studies and can answer the following concerns:

What is the background DNA contamination like on these swabs?

How does the yield from these swabs compare to extracting directly from a swab with no buffer (im concerned about the dilution effect of 1mL of buffer)?

Hoping any answers will guide me as to whether I have to go back to the beginning with designing my protocol!

Thanks

Alison

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