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More Sepideh Moradkhani's questions See All

How can I find the equilibrium time for ODE functions?

I have an ODE function with 5 variables that is complicated to solve theoretically. Therefore, I solved it numerically and found the equilibrium point (values for the variables). Is there an easy...

01 July 2024 5,223 3 View

Hello dear researchers Is there an alternative for gold chloride salt in electrochemical biosensor studies?

The price of gold chloride salt is unfortunately very high.

08 June 2024 1,469 0 View

I have question about transduction with pCDH-CMV-MCS-EF1-CopGFP-T2A-Puro vector?

Greetings, recently I have acquired the pCDH-CMV-MCS-EF1-CopGFP-T2A-Puro vector (V012884). I would like to know whether the gene of interest's expression is regulated by the CMV promoter or not,...

22 May 2024 8,128 2 View

Why we trim developed flowers before Arabidopsis transformation, is it because they cannot be transformed?

I am working with a flowering mutant, so the transformation is not so easy, I need to repeat it every 7 days, but I was wondering what will happened to the flowers if I dip them again in...

01 May 2024 7,141 2 View

Why is the NiFe MOF not put on the nickel foam?

I have seen type of MOF powder perfectly 7:3 Ni/Fe by solvothermal method how ever when I put Ni foam in the autoclave and the same I can not have the appropriate MOF coating on that foam. I...

16 April 2024 6,554 0 View

How long a transformed bacteria with correct 3HR and 5HR assembly can be stored in fridge?

I cloned 3HR and 5HR of p.falciparum on a plasmid and the next step is gRNA cloning. I did colony PCR for verification it is perfect but the thing is after taking the toothpeak of the colony in...

12 March 2024 5,716 2 View

Why do I get three bands after restriction digestion?

I ran restriction digestion on my plasmid just to verify my inserts and send for sequencing. my backbone plasmid is PL6 with 9843 bp containing a 3HR (877bp) and 5HR (782). My enzymes for 3HR is...

07 February 2024 8,015 4 View

How to test (CCS & MOR) refractory special bricks?

Dear researchers and experts, I've been investigating different testing methods for refractory bricks with special shapes. There are a few methods and standards for testing refractories; I wonder...

07 January 2024 831 3 View

What is the best bioinformatics software that can tell us the relationship between LNCRNA and microRNA?

I checked a lot of software like DIANA Tools, Sponge Scan, Mirtat Target Scan, etc., but unfortunately, I did not get any results.

29 November 2023 1,899 0 View

Hello. Does anyone have a suggestion for a research project on children's ALL?

I need a research project on pediatric ALL please help me

25 October 2023 2,528 4 View

How to learn more about SPSS and its Application?

I would like to learn more about SPSS and Its application especially in regards to data analysis. Please suggest me how I can learn more about it. Thank you so much.

11 August 2024 9,101 4 View

How to confirm the site-directed mutagenesis result without performing NGS?

I'm cloning a fragment of 3200 nts into plasmid. The cloning was successful, however, 02 amino acids were mutated. Now I want to fix these 02 aa by site-directed mutagenesis technique using...

08 August 2024 4,645 2 View

I can't see the ssDNA band after performing asymmetric PCR. Is there any way to do this?

After performing symmetric PCR, PCR purification was performed. Afterwards, asymmetric PCR was performed using the PCR purification product as a template, but no ssDNA band was confirmed in the...

08 August 2024 1,668 3 View

Are there any instruments for studying time similar to the way it is in space?

There are a huge number of methods for studying objects in space, according to the senses (and not only). Mechanical, thermal, optical, acoustic, electrical, magnetic, based on particle beams,...

06 August 2024 7,102 0 View

How much total RNA concentration to be extracted from sorted plasma cells from bone marrow of C57BL/6 mice for RT-PCR ?

i have sorted anti-NP specific plasma cells from bone marrow of C57BL/6 mice at certain times after immunization with variable counts and isolated total RNA using TRIZOL method for RT-PCR using...

05 August 2024 8,835 1 View

"A Markov-like Model for Patient Progression"?

A Markov-like Model for Patient Progression" Markov Chain Monte Carlo (MCMC) Markov Chain Monte Carlo (MCMC) is a powerful computational technique used to draw samples from a probability...

05 August 2024 10,079 0 View

Why does the MFDFA algorithm need to calculate the profile of the time series?

As described in the Multifractal detrended fluctuation analysis (MFDFA) algorithm, it at first calculates the profile of the time series, and then other steps are operated on the profile....

05 August 2024 9,366 2 View

Why after performing site directed mutagenesis ,I don't see any colony after transformation?

I want to introduce a point mutation (change in one nucleotide) into my gene of interest (DNA binding domain) I have designed primers as recommended on the Data sheet of the kit : -Both primers...

05 August 2024 9,059 3 View

If we are using snowball sampling technique, how do we justify the true representativeness of the sample statistically? is there any statistical test?

Are there any statistical methods to justify your sampling technique using SPSS or AMOS?

05 August 2024 9,153 4 View

Why my colony PCR results of my recombinant bacterial not showing any results?

I am performing ligation of the plasmid and a target gene. The steps I have taken are: 1. Double digestion of the plasmid and target gene 2. Ligation of the plasmid with the target gene 3....

05 August 2024 2,570 3 View