1picomole/ul= uM

Hi Jawad, I guess you want to run PCR reaction and what you mentioned are initial (stock) concentrations? If yes, what is the final concentration of each? What is the final volume of your reaction? Please, provide me these information so I can help you.

Good luck

Ahmed

Jawad, check this link which might help you

https://www.neb.com/protocols/0001/01/01/protocol-for-a-routine-taq-pcr-reaction

Hello,

if you have the original concentrations of your reagents, then you need to decide the final volume of your reaction. I usually run 20μl reactions when I am setting things up, and once my conditions are set I scale my reactions to 50μl.

To calculate the volumes for each reagent I use the following formula:

C1xV1=C2xV2

Where

C1 is the concentration of your stocks

V1 is the unknown volume you need to calculate

C2 is the concentration in the reaction

V2 is the final volume of your reaction.

I also use molecular gra water to reach the final volume.

The only thing that you really need to be careful about is the unites.

Hope the answer helps!

Kamal Kumar , Ahmed Dhamad and Olga Biskou

I really appreciate your effort too much

thanks a million for your wonderful help

again, many thanks for your kind

jawad