How to find full length sequences of MYB genes of wheat?

Hi, How to find full length sequences of MYB genes of wheat?

08 September 2016 8,710 7 View

How to design gene specific primers?

06 July 2016 2,231 9 View

Is it RNA?

Hi, I extract RNA from leaf under normal & different stress condition, and synthesize cDNA but when I check its expression level with tubulin , untreated leaf give me normal result as show in...

05 June 2016 2,349 11 View

Gene expression under stress conditions?

Hi every one, I have a question that any body use PEG, NaCl and cold stresses to check gene expression under these stress conditions for checking expression level and what kind of tissues used e.g...

04 May 2016 4,724 11 View

Any one use QIAGEN RNase free DNase set (79254)? What about it results?

03 April 2016 5,397 5 View

Why my RNA always degrade?

Hi, I extract RNA from different plant tissues using Trizole as well as Qiagen RNAEasy kit but when ever i extract RNA by these two methods my RNA always degrade and have genomic DNA traces....

03 April 2016 4,515 4 View

How can I equalize RNA concentration of all samples?

03 April 2016 4,265 9 View

How to design primers?

02 March 2016 9,436 5 View

Which step is important in Primer designing?

Hi everybody, I want to know the critical steps in designing primers, means what will suitable Tm value, GC content, false priming, dimer, cross dimer, hairpin, rating, length of primer, product...

02 March 2016 6,727 3 View

Is degraded RNA converted to cDNA by RT PCR using reverse transcriptase?

02 March 2016 7,156 7 View

Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence?

I am on the lookout for the Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence. Does anyone know where I can find it? Thank you in advance

03 March 2021 3,568 1 View

Does anyone know HRM channel in a 2 plex QIAGEN Rotor- Gene Q qPCR can be used as a separate channel?

I am going to have 3 different probes in my qPCR work that I am going to do. But I realized that the machine we have in the lab is a Rotor-Gene Q 2plex HRM Platform, saying it has green, yellow,...

01 March 2021 8,544 1 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

The reason that the culture of white fungi Contamination with green mold(Trichoderma), the culture autoclave at121 for 15 min. What is it?

Is the period to autoclave not enough? The inoculation in a hood and flame and UV and alcohol.

27 February 2021 9,356 3 View

Calculating volume of DNA required from DNA concentration?

Hi I am a bit confused. They are asking me to find out the volume of DNA required in ul (a total of 30-100 ng for genomic DNA) from the DNA concentration in the nanodrop reading which was 404.8...

26 February 2021 5,029 2 View

Has anyone used the Illumina ITS1 primer sets?

Hi everyone, Illumina provides a list of primers to amplify with high taxonomic coverage the ITS1 region for further fungal sequencing, but I cannot find the exact amount necessary of each...

25 February 2021 6,969 3 View

Errors in DNA sequences?

I am trying to better understand the scope of DNA replication and sequencing errors, e.g. 1. I have seen similar error rates of 10e4 to 10e5 for cell & instrumental DNA replication,...

24 February 2021 4,397 3 View

What might be the reason for obtaining two bands in plant genomic DNA extraction?

I got a thin band and a thick band i guess it would be the genomic DNA and the 260/280 ratio is 3.

21 February 2021 6,523 6 View

Color of spiro-ometad film?

What does the color of a spiro-ometad film indicate? I got purple, green, red, even black color of the spiro film. Is there anything we can get from the preliminary color analysis(e.g. oxidation...

21 February 2021 1,831 4 View

I can detect my protein but the encoding DNA sequence has stop codons within. Should I ignore the stop codons?

I constructed a synthetic DNA library (scFv, VH-VL orientation) with a 3' reporter and 8x histag. I cloned and expressed this gene following which I performed an ELISA. The ELISA results suggested...

19 February 2021 7,006 5 View