19 Questions 41 Answers 0 Followers
Questions related from Bushra Khattak
Hi, How to find full length sequences of MYB genes of wheat?
09 September 2016 8,782 7 View
Hello, I want to know, How to design gene specific primers for A, B & D genome in case of wheat ? What about annealing temp & cycles use to amplify perfectly?Thanks
07 July 2016 2,287 9 View
Hi, I extract RNA from leaf under normal & different stress condition, and synthesize cDNA but when I check its expression level with tubulin , untreated leaf give me normal result as show in...
06 June 2016 2,397 11 View
Hi every one, I have a question that any body use PEG, NaCl and cold stresses to check gene expression under these stress conditions for checking expression level and what kind of tissues used e.g...
05 May 2016 4,778 11 View
Hi, I extract RNA from different plant tissues but RNA concentration when check on nanodrop varies too much from each other e.g one sample concentration is 3066 ng/ul and other is 6000 ng/ul but...
04 April 2016 4,327 9 View
Hi, I extract RNA from different plant tissues using Trizole as well as Qiagen RNAEasy kit but when ever i extract RNA by these two methods my RNA always degrade and have genomic DNA traces....
04 April 2016 4,571 4 View
Hello, I have two questions here. 1st is it important to equalize RNA concentration for cDNA synthesis in case of gene expression? I do calculation using formula Vol1 *conc1= Vol2 *conc2 , but i...
04 April 2016 5,450 5 View
Hi everybody, I want to know the critical steps in designing primers, means what will suitable Tm value, GC content, false priming, dimer, cross dimer, hairpin, rating, length of primer, product...
03 March 2016 6,778 3 View
Hi, I want to know how to design primer pair for A, B and D genome of wheat together, in order to check expression level in different organ?
03 March 2016 9,501 5 View
Hi, I want to ask if degraded RNA is able to synthesize cDNA by RT PCR? If yes, so when we use this cDNA and do PCR for cloning, so it affect original pcr product size etc? I want to clone one...
03 March 2016 7,212 7 View
Hi, Can any one tell me how to convert DNA in microgram into microliter? For example how much 2 micro gram of DNA equal to how much microliter? Please show with formula
01 January 2016 4,489 1 View
Hi, I transform my gene of interest+ MYC into blunt end vector (my gene of interest+ MYC is sticky end with SpeI) so for doing colony PCR can I use blunt end vector Reverse & Forward primers...
12 December 2015 7,510 4 View
Hello,any one here working on MADS box genes of brachypodium and wheat?I want to know is it important that MADS box genes must have MIKC domains,or some time missing of one domain cause problem or...
12 December 2015 4,083 2 View
Hi, Can any one help me how to draw below vector in ppt, i tried a lot but no success. Thanks
11 November 2015 8,503 1 View
Hi, I am really in trouble, I spend almost 3 months on constructing C Terminal vector with 4 MYC but always after sequencing and alignment i get wrong in MYC sequence, some mutations. MYC sequence...
11 November 2015 5,067 17 View
Hii everyone. I have a problem in case of pGEM T vector. I ligate my gene of interest and tag together into pGEM T vector but after transformation i get no colonies. I followed this protocol for...
11 November 2015 2,574 1 View
Hi, i want to know can we avoid use of LA taq polymerase because of not proofreading capability for ligation? Can we use another vector which is use for proofreading taq polymerase? Please suggest...
11 November 2015 7,530 4 View
HI, How much 1 micro gram, nano gram,pico gram, ng/ul DNA equal to microliter?e.g 200ng DNA is equal to how much microliter?How we calculate it?
10 October 2015 1,597 10 View
Hi, I want to ask why don,t we use PCR product directly in cloning, why we prefer to extract DNA in every step? Secondly when ever i extract DNA from gel its concentration is very low, around...
10 October 2015 7,008 15 View
