Hello, I want to know, How to design gene specific primers for A, B & D genome in case of wheat ? What about annealing temp & cycles use to amplify perfectly?Thanks
Hi, i think the following site will be helpful......best
http://projects.nfstc.org/pdi/Subject04/pdi_s04_m01_02.htm
A great tool for designing gene/species/position specific primers. The end result is a PDF with all specifications that you need.
http://genepipe.ncgm.sinica.edu.tw/primerz/beginDesign.do
@Julia Pazmandi, I think this tool is for animals and human not for plants. Can you please suggest me same tool for plants e.g wheat, rice. Thanks
http://www.bioinformatics.nl/cgi-bin/primer3plus/primer3plus.cgi here you paste any sequence that your interested in (your gene of interest in your case ). You'd have to paste the nucleotide sequence of your whole gene in, hence it does not differentiate between.
If this isn't what your looking for, here is a summary of the online primer design tools available:
http://molbiol-tools.ca/PCR.htm.
You're welcome! I discovered it in these days.. after two years of manually designing more or less. In Italy we say "better late than never" :D
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