How to find full length sequences of MYB genes of wheat?

Hi, How to find full length sequences of MYB genes of wheat?

08 September 2016 8,710 7 View

Is it RNA?

Hi, I extract RNA from leaf under normal & different stress condition, and synthesize cDNA but when I check its expression level with tubulin , untreated leaf give me normal result as show in...

05 June 2016 2,349 11 View

Gene expression under stress conditions?

Hi every one, I have a question that any body use PEG, NaCl and cold stresses to check gene expression under these stress conditions for checking expression level and what kind of tissues used e.g...

04 May 2016 4,724 11 View

Any one use QIAGEN RNase free DNase set (79254)? What about it results?

03 April 2016 5,397 5 View

Why my RNA always degrade?

Hi, I extract RNA from different plant tissues using Trizole as well as Qiagen RNAEasy kit but when ever i extract RNA by these two methods my RNA always degrade and have genomic DNA traces....

03 April 2016 4,515 4 View

How can I equalize RNA concentration of all samples?

03 April 2016 4,265 9 View

How to design primers?

02 March 2016 9,436 5 View

Which step is important in Primer designing?

Hi everybody, I want to know the critical steps in designing primers, means what will suitable Tm value, GC content, false priming, dimer, cross dimer, hairpin, rating, length of primer, product...

02 March 2016 6,727 3 View

Is degraded RNA converted to cDNA by RT PCR using reverse transcriptase?

02 March 2016 7,156 7 View

Conversion og microgram into micro liter?

Hi, Can any one tell me how to convert DNA in microgram into microliter? For example how much 2 micro gram of DNA equal to how much microliter? Please show with formula

31 December 2015 4,445 1 View

In PRIMER, is it possible to downweight certain taxa in multivariate community datasets to reflect uncertainty around identification??

I have a dataset with about 80 different species. As usual, some species are very easy to identify with certainty whereas others are more difficult, which means that I am less certain of my...

03 March 2021 8,066 4 View

No title

02 March 2021 3,060 3 View

How to calculate BREQ 3 questionnaire?

how to calculate BEHAVIOURAL REGULATION IN EXERCISE QUESTIONNAIRE BREQ 3 ,which contain 24 questions and each question has 5 answers (0- Strongly disagree , 1-disagree, 2- Neither agree nor...

02 March 2021 811 3 View

What do you know about biotechnology art?

What do you know about biotechnology art?

02 March 2021 1,653 3 View

Which is the best place in the world for the plant biotechnology research?

I am searching for a good place for the Post Doc,

02 March 2021 4,053 3 View

PACYC PCR not working after several attempts. Would anyone have any suggestions?

So, I have been trying to run a pACYC PCR which will be used later on for a Gibson Assembly. However the PCR is not working. I have already tried gradient PCR and changing extension time; however...

02 March 2021 1,146 2 View

I am interested in medicinal chemistry field, which books are highly recommended to learn from?

I am at the beginner level in drug design and drug discovery, and I am interested to learn more about this field. Which books are highly recommended to start from and gives me all essential...

02 March 2021 8,437 2 View

Please suggest a good text book for "Research Methodology in Life Sciences". Which book would be good for PhD Scholars?

Please provide Book Title and author name

02 March 2021 9,059 3 View

Could any one guide me, to write research proposal for grant? ?

We have one plant, the local people using this for controlling blood sugar, the toxicity and antidiabetic activity of the plant is not reported yet, please guide from whag should be the process ?...

02 March 2021 2,300 1 View

How to calculate the annealing temperature of a primer pair?

I have to amplify a gene and my primers just reached. The Tm for Forward primer is 64.2, and that of reverse primer is 65.5. Can some one suggest how to get the best annealing temperature? Thanks...

01 March 2021 360 7 View