Does anyone know good conference in Europe neurodegeneration or aging, which starts in 2014 September-October ?
Hi I would like to develop some medical assays for example for cancer prevention, or analysis of some markers for some disease which would be good for application in medicine in Georgia country....
04 May 2019 5,102 3 View
I did synthesis of 2 peptides (1-20 amino acids) using MDHA Rink resin, I used HBTU (3.7g in 20ml (DMF)), HOBT (1.35g (29ml) NMP) to get 0.5 M. For each reaction I used 1 mL HBTU solution, 1ml...
10 November 2018 7,622 4 View
Hi all I am interesting if you want to produce some protein and have plasmid, the way is to grow it in bacteria and than isolate with antibody which will bind tag (example FLAG tag etc.) but what...
09 October 2018 8,696 5 View
How to view an article short term if journal is not open access? Is there any website which for example shows content but is not possible to copy?
07 August 2018 10,206 3 View
I am interesting if somebody can suggest in Excel 2016, I have columns and want to write mine values instead this it chooses only automatic range for example from 1 to 40. I want type for example...
07 August 2018 7,341 0 View
I want to study if bacterias are associated with root, can anyone suggest for fluorescent marker which would be better to use? or which method is good (example some staining solution like acridine...
11 December 2017 2,391 5 View
Hi All There is opening laboratory of molecular biology in Georgia country. I am interesting which kind of product can produce (budget 10 000 dollars) molecular biology laboratory to start...
09 October 2017 9,743 0 View
I have some mix of bacteria and would like to know which species there are exactly. Should I send them for sequencing their DNA? or what would be cheap method?
06 July 2017 9,692 10 View
Does anyone know any website which lets at least just to read article of journals which have not open access? if its not possible to download a PDF.
09 October 2016 4,346 4 View
I am interesting can anyone suggest how to track protein transport into the cells not using GFP or any big markers? I would like to minimize the effect of marker (keep size and function of protein...
08 September 2016 6,918 7 View
I have virus (viral hemorrhagic septicemia virus) in suspension and the experiment will not involve cells. What level of TCID50 is preferred?
11 August 2024 3,115 1 View
I have carried out MFC experiments on three different volumes, 50, 500 and 1000 mL of wastewater. Results after MFC treatment shows that TDS and EC are more in larger volumes of water i.e. TDS and...
09 August 2024 9,621 0 View
I am using Rhodamine6G as gain medium and silver nanoparticles as scatterers on a microscope slide and laser input 532 nm comes from above.
09 August 2024 9,894 2 View
Hello everyone! I observed in my culture (htert-RPE1 cells) an orange- red particle at the bottom of the dish. It is visible to the naked eye as a very very small red dot. Could it be a...
09 August 2024 2,824 3 View
I'm cloning a fragment of 3200 nts into plasmid. The cloning was successful, however, 02 amino acids were mutated. Now I want to fix these 02 aa by site-directed mutagenesis technique using...
08 August 2024 4,645 2 View
After performing symmetric PCR, PCR purification was performed. Afterwards, asymmetric PCR was performed using the PCR purification product as a template, but no ssDNA band was confirmed in the...
08 August 2024 1,668 3 View
I am working in fungal fermentation of soybean meal and there is bacterial growth in them at times. I am trying to quantify fungal cell counts and bacterial cells; but I haven't been able to do at...
07 August 2024 7,535 4 View
Hello dear colleagues, We have prepared a manuscript on NiTi-based alloys and are seeking a second opinion on our current TEM results. If you are a Ph.D. holder with experience in TEM and have...
07 August 2024 9,563 0 View
Hi, I have a question about normalizing the MTT OD values for doing the statistical analysis. So, if we have 3 different plates and we call them 3 different replicates, so, first we would...
07 August 2024 8,106 4 View
Previously when I co-coluture anti-CD19(FMC63) CAR-Jurkat with Raji with E:T=5:1, Jurkat can eliminate Raji in 24h. However, when I test another CAR construct, although I can dectect totally CD69...
06 August 2024 641 2 View