Does soft algniate hydrogel influence calcium concentration of a solution (cell culture medium)?

05 January 2021 6,066 4 View

Why does a magnetic mirror acting on ballistic electrons not violate the 2nd law of thermodynamics?

Magnetic mirrors are well known in plasma physics. In order to work, the mean free path of the charge carriers has to be at least as long as the helical paths under the influence of the B field....

24 October 2020 8,124 3 View

Collagen Type I Hydrogel dissociates/degradates/desintegrates in culture with cells. Where am I going wrong?

Hi all, I am having trouble with collagen type I as a scaffold for cells. I have a 3mg/mL collagen I solution with pH = 2. I am neutralizing with 1 M NaOH to pH ~7, then mixing with ice cold...

22 October 2020 8,429 3 View

Magnetic monopole pairs generated by photon/photon collisions?

The transformation of two "colliding" photons into a pair of particle / anti-particle was experimentally observed already. But the observed particles are always electrically charged. 1. If such a...

30 July 2019 1,569 18 View

Does PDMS cure when it is in contact to water?

I want to seal a tissue/glass interface. The tissue should not be out of the water (buffer) for too long, so I am wondering if I could apply PDMS in liquid form and let it cure under water?...

17 October 2016 3,563 7 View

Which optical E-field sensor would you recommend for time-domain measurements?

For some years, optical E-field sensors (plus remote units) are commercially available; main advantage: the field is much less disturbed than by electrically conductive sensors. We would like to...

21 April 2016 3,688 5 View

Should we use 5-pt or 7-pt Likert scales? Any impact on significance?

Hello everyone A student of mine recently asked if it matters for significance testing whether a 5-pt or a 7-pt Likert scale is used. That is, are effects more likely to become statistically...

13 April 2015 5,188 9 View

Volition in music - Seeking for international colleagues for further development of a volitional online test - interested?

The "will test in music" is available as volitional test for German speaking music students. This test should be provided in different language versions. In a first step, I would like to make an...

27 December 2014 1,946 10 View

Can anybody recommend a good software package for analyzing multilevel SEM?

I need to test an SEM model that involves multiple levels of analysis (individual, group, organization) and I am currently looking for an appropriate software package that allows me to do so. If...

30 January 2014 5,404 25 View

Anyone want to collaborate on a data base for animated organic chemical reaction mechanisms?

I started to develop a data base with gif-files of organic reaction mechanisms. Are there any other colleagues who are working in this field and want to...

28 January 2014 8,121 2 View

Buffer to disrupt platelet alpha granules for PF4 quantification?

Hello! I want to quantify by ELISA the secreted (from platelets poor plasma) and the non-secreted (from platelet lysate) PF4 before and after TRAP stimulation. I will use the ELISA from R&D...

03 March 2021 1,499 2 View

Buffer Exchange using column packed with sephadex G-25 effect on protein concentration?

Hello, If i am doing a buffer exchange for an antibody of 1mg/mL, does the elution lose protein in the process of buffer exchange? For example, if i flow through 500 uL of 1mg/mL sample, and...

03 March 2021 6,299 3 View

How to increase a model accuracy ?

dear community, my model is based feature extraction from non stationary signals using discrete Wavelet Transform and then using statistical features then machine learning classifiers in order to...

03 March 2021 6,994 5 View

Gel electrophoresis result for total RNA samples, 1.5 Agarose gel was used, How to improve the integrity of RNA?

Gel electrophoresis, RNA degradation, RNA extraction from fresh tissue

02 March 2021 5,433 5 View

How optimize the Size Exclusion Chromatography?

Hello! I'll do a size exclusion chromatography, but I only have an open column, and I'll perform the peptide extraction from yeast, using buffer lysis (sodium phosphate 50 mM/NaCl 30 mM/DNAse and...

01 March 2021 2,215 2 View

Why are my bands weird in this Agarose gel?

Can someone please give me some possible things that could go wrong? Here is my recipe: 0.5g Agarose 50 mL of TAE 1x 1 uL ethyl bromide. Gel was run at 100V for 1 hour. The buffer used is also TAE.

01 March 2021 9,952 3 View

What do I do with DLS peaks that are from the buffer?

I'm looking at the aggregation of my protein sample using DLS. Unfortunately, my buffer (20mM HEPES) also results in a set of peaks. These are at approximately 1 and 1000 d.nm. The lowest peak...

01 March 2021 9,015 2 View

Problem with shRNA transfection. Why are only two plates getting contaminated instead of all?

I transfected my LNCaP-WT cells with 3 shRNA plus their NTC two weeks ago and split two puromycin selected cell plates on Friday last week(Feb 26). I checked for GFP in the cells, and they all...

28 February 2021 4,949 3 View

Any specific pipeline or parameter to analyse total transcriptomics study of brain tissue samples?

I have two groups of brain samples, control and treated for example. It was total RNA nova seq sequencing. I tried all the available pipeline like: star+rsem+deseq2, Hista+stringtie+cuffdiff,...

27 February 2021 356 6 View

Why is the efficiency of viral mRNA electroporation in PBMC low?

Hello, I have been struggling with the electroporation of PBMC with HIV-I mRNA. Although the quality of the RNA generated is good, and the handling of the cells in meticulous, the frequency of...

27 February 2021 9,233 3 View