Does soft algniate hydrogel influence calcium concentration of a solution (cell culture medium)?

05 January 2021 6,066 4 View

Why does a magnetic mirror acting on ballistic electrons not violate the 2nd law of thermodynamics?

Magnetic mirrors are well known in plasma physics. In order to work, the mean free path of the charge carriers has to be at least as long as the helical paths under the influence of the B field....

24 October 2020 8,124 3 View

Collagen Type I Hydrogel dissociates/degradates/desintegrates in culture with cells. Where am I going wrong?

Hi all, I am having trouble with collagen type I as a scaffold for cells. I have a 3mg/mL collagen I solution with pH = 2. I am neutralizing with 1 M NaOH to pH ~7, then mixing with ice cold...

22 October 2020 8,429 3 View

Magnetic monopole pairs generated by photon/photon collisions?

The transformation of two "colliding" photons into a pair of particle / anti-particle was experimentally observed already. But the observed particles are always electrically charged. 1. If such a...

30 July 2019 1,569 18 View

Concentration of TCEP for RNA Isolation?

Hi all, in our lab we would like to switch from Mercaptoethanol to TCEP as a reducing agent for RNA protection during isolation. What concentration do you use? I only found a protocoll from...

01 March 2017 4,234 2 View

Does PDMS cure when it is in contact to water?

I want to seal a tissue/glass interface. The tissue should not be out of the water (buffer) for too long, so I am wondering if I could apply PDMS in liquid form and let it cure under water?...

17 October 2016 3,563 7 View

Which optical E-field sensor would you recommend for time-domain measurements?

For some years, optical E-field sensors (plus remote units) are commercially available; main advantage: the field is much less disturbed than by electrically conductive sensors. We would like to...

21 April 2016 3,688 5 View

Should we use 5-pt or 7-pt Likert scales? Any impact on significance?

Hello everyone A student of mine recently asked if it matters for significance testing whether a 5-pt or a 7-pt Likert scale is used. That is, are effects more likely to become statistically...

13 April 2015 5,188 9 View

Volition in music - Seeking for international colleagues for further development of a volitional online test - interested?

The "will test in music" is available as volitional test for German speaking music students. This test should be provided in different language versions. In a first step, I would like to make an...

27 December 2014 1,946 10 View

Can anybody recommend a good software package for analyzing multilevel SEM?

I need to test an SEM model that involves multiple levels of analysis (individual, group, organization) and I am currently looking for an appropriate software package that allows me to do so. If...

30 January 2014 5,404 25 View

Why does blocking with my immunogenic peptide cause nuclear fluorescence in ICC?

Hi all, I am validating a polyclonal antibody raised against human ANT4, through WB and ICC. In most of my controls, the antibody shows very little off-target binding, but when I pre-incubate the...

03 March 2021 5,999 3 View

Have someone successfully used the ER alpha (Santa Cruz sc-8002) antibody in immunostaining ?

Santa Cruz now recommends this new antibody (F-10) but I can't find a publication using this reference. I'm working on human et non human primate hypothalamus and so far, it doesn't work. Is...

02 March 2021 10,003 2 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

Does anyone know an alternative product for cardiac Troponin T antibody for Immunofluorescence (Thermo # MA512960)?

We have been using Thermo Fisher anti-cardiac Troponin T antibody for immunofluorescence staining in FFPE mouse heart sections but it was recently discontinued. The literature shows it to be the...

01 March 2021 4,937 1 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

How does an antibody have full intact antibody + half antibody?

Hello, I was just wondering how does a antibody have full intact antibody + half antibody? Is it because of the drug conjugation?

28 February 2021 1,577 1 View

Does anyone use antinuclear antibody (ANA) and anti-Phospholipid antibody ELISA kit for Non Human Primate serum samples?

I am looking for anti-nuclear antibody (ANA) and anti-Phospholipid antibody ELISA kit for Non Human Primate serum samples. Any suggestions highly appreciated. Thank you.

28 February 2021 6,512 3 View

RIA. How to measure?

When you use RIA, with a control the unknown sample with antibodies, you bare in mind the binding sites of the antibodies. However you still need to measure labelled antigen (radioactive) and not...

28 February 2021 2,133 3 View

Anyone know of a good progesterone antibody?

I am looking for a good progesterone antibody to quantify levels of progesterone in zebrafish. Applications would be ELISA, and immunohistochemistry.

28 February 2021 5,929 2 View

Will a 10xHis-tag prevent anti-MBP from binding to MBP?

I have a protein with a 10xHis-MBP tag on the N-terminus. I'm fairly certain I am expressing my protein, but I'm not getting a signal on my western blot. Is there a possibility that the 10xHis tag...

25 February 2021 8,404 3 View