Does soft algniate hydrogel influence calcium concentration of a solution (cell culture medium)?

05 January 2021 6,066 4 View

Why does a magnetic mirror acting on ballistic electrons not violate the 2nd law of thermodynamics?

Magnetic mirrors are well known in plasma physics. In order to work, the mean free path of the charge carriers has to be at least as long as the helical paths under the influence of the B field....

24 October 2020 8,124 3 View

Collagen Type I Hydrogel dissociates/degradates/desintegrates in culture with cells. Where am I going wrong?

Hi all, I am having trouble with collagen type I as a scaffold for cells. I have a 3mg/mL collagen I solution with pH = 2. I am neutralizing with 1 M NaOH to pH ~7, then mixing with ice cold...

22 October 2020 8,429 3 View

Magnetic monopole pairs generated by photon/photon collisions?

The transformation of two "colliding" photons into a pair of particle / anti-particle was experimentally observed already. But the observed particles are always electrically charged. 1. If such a...

30 July 2019 1,569 18 View

Concentration of TCEP for RNA Isolation?

Hi all, in our lab we would like to switch from Mercaptoethanol to TCEP as a reducing agent for RNA protection during isolation. What concentration do you use? I only found a protocoll from...

01 March 2017 4,234 2 View

Does PDMS cure when it is in contact to water?

I want to seal a tissue/glass interface. The tissue should not be out of the water (buffer) for too long, so I am wondering if I could apply PDMS in liquid form and let it cure under water?...

17 October 2016 3,563 7 View

Which optical E-field sensor would you recommend for time-domain measurements?

For some years, optical E-field sensors (plus remote units) are commercially available; main advantage: the field is much less disturbed than by electrically conductive sensors. We would like to...

21 April 2016 3,688 5 View

Should we use 5-pt or 7-pt Likert scales? Any impact on significance?

Hello everyone A student of mine recently asked if it matters for significance testing whether a 5-pt or a 7-pt Likert scale is used. That is, are effects more likely to become statistically...

13 April 2015 5,188 9 View

Volition in music - Seeking for international colleagues for further development of a volitional online test - interested?

The "will test in music" is available as volitional test for German speaking music students. This test should be provided in different language versions. In a first step, I would like to make an...

27 December 2014 1,946 10 View

Can anybody recommend a good software package for analyzing multilevel SEM?

I need to test an SEM model that involves multiple levels of analysis (individual, group, organization) and I am currently looking for an appropriate software package that allows me to do so. If...

30 January 2014 5,404 25 View

How to compare two groups with only two measurements?

Hiiiii everyone! I have an enquiry on statistical analysis. I was looking for many forum and it's still cannot solve my problem. I want to compare means of two groups of data but only with two...

03 March 2021 8,796 3 View

Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence?

I am on the lookout for the Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence. Does anyone know where I can find it? Thank you in advance

03 March 2021 3,568 1 View

What kind of fluid could I use for a pitfall trap that also does not invalidate molecular testing?

Hi, I want to start testing pitfall trap to obtain ants samples, but I need to conduct molecular analysis on those insects. So, what kind of fluid can I use? Ethanol expires too early and I need...

03 March 2021 5,978 5 View

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10?

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10? Since, the growth curve from day 2 to 10 won't be like the "Logistic curve" it might not follow logistic...

03 March 2021 1,401 3 View

Do we need to write protocol for a second study of a systematic review that has been registered and published?

I have conducted and published a systematic review and meta-analysis research with the topic related to public health and health pomotion (protocol was registed in PROSPERO). Now we want to...

03 March 2021 8,920 3 View

How to increase a model accuracy ?

dear community, my model is based feature extraction from non stationary signals using discrete Wavelet Transform and then using statistical features then machine learning classifiers in order to...

03 March 2021 6,994 5 View

Do I need to do linear regression before running mediation analysis using PROCESS MACRO by Hayes ?

I just wanted to check if I need to run a linear regression separately if I am using PROCESS MACRO to run mediation analysis. Thank you.

02 March 2021 4,359 3 View

Sample dilution for quantitative analysis using ELISA?

If the detection range is in ng/ml but the reference range is in ug/ml for a molecule or protein in serum or plasma .how to dilute and what is the initial volume to be taken for quantitative analysis

02 March 2021 7,670 3 View

Is There Any Feasible Method To Test Fluorescent Compounds Other Than Fluorescence Spectrometers ??

Is There Any Feasible Method To Test The Efficiency Of Fluorescent Compounds Other Than UV Spectrometers ? Suggestions Would Be Appreciated !

02 March 2021 5,785 3 View

How to calculate average trend in maximum annual NDVI from MODIS?

I am wanting to calculate the average trend in maximum annual NDVI in Iceland from 2010-2020 using MODIS MYD13Q1 V6. How would I do this? I have currently inserted the NDVI bands from the MODIS...

02 March 2021 752 2 View