Jörg Panzer

5 Questions 11 Answers 0 Followers

Questions related from Jörg Panzer

IF-Antibodies to discriminate between smooth muscle actins alpha- and gamma-SMA?

Hi everyone, I want to analyse the differentiation status of primary intestinal smooth muscle cells. One thing that I want to investigate is the expression of the two smooth muscle specific actin...

13 January 2022 4,070 2 View

Does soft algniate hydrogel influence calcium concentration of a solution (cell culture medium)?

Hi everybody, I am wondering if the unoccupied Ca2⁺ binding sites in soft alginate gel (gelation with low Ca2+ concentration) sequestrate the free Ca2+ of my cell culture medium. Which would also...

06 January 2021 6,204 4 View

Collagen Type I Hydrogel dissociates/degradates/desintegrates in culture with cells. Where am I going wrong?

Hi all, I am having trouble with collagen type I as a scaffold for cells. I have a 3mg/mL collagen I solution with pH = 2. I am neutralizing with 1 M NaOH to pH ~7, then mixing with ice cold...

23 October 2020 8,556 3 View

Concentration of TCEP for RNA Isolation?

Hi all, in our lab we would like to switch from Mercaptoethanol to TCEP as a reducing agent for RNA protection during isolation. What concentration do you use? I only found a protocoll from...

02 March 2017 4,388 2 View

Does PDMS cure when it is in contact to water?

I want to seal a tissue/glass interface. The tissue should not be out of the water (buffer) for too long, so I am wondering if I could apply PDMS in liquid form and let it cure under water?...

18 October 2016 3,685 7 View