I'm not sure how DNAi is best transfected (it's a recent thing after all) but you would do yourself well by starting out with the normal concentration for plain DNA in transfections (see example concentrations for a protocol here: https://altogen.com/product/nanoparticle-in-vivo-transfection-reagent/ ). Depending on the effects you're seeking, you'll have to modulate the concentration until you reach an acceptable overall efficiency.

Hello Ted

I was thinking the same, perhaps I should try it similar as a siRNA then see what happens. Thank you.

Hi, you can try with different concentrations of DNAi or SiRNA for 24h, 48h or 72 hours. Then you may check which concentration or time shows best result by western blot. You might try with different transfecting solution if anyone doesnt work well. In the case of some cell-line, some specific transfecting reagent works fine. In that case you might check some published papers regarding that cell-line transfection. Thanks and well wishes.