Codon optimization of human gene for human host. Does it make sense?

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Does anyone know why this article is republished using the fake address. Is it about stealing my patent?

This article is mine. It was republished by unknown person using an address (Wilson N Jones Regional Center, Sherman Texas USA. I have a patent.

10 August 2024 7,528 5 View

Aspen Plus Simulation?

Greetings When one is to perform Simulation or modelling of Pyrolysis of Biomass Waste materials using Aspen Plus software, and one has both the macromolecules breakdown (Cellulose, Lignin and...

06 August 2024 5,023 1 View

Evaluating a developed cybersecurity framework for SMEs in Nigeria?

I am humbly seeking an expert to evaluate my developed cybersecurity framework on my Master degree final project titled " Developing a framework to Enhance Organizational Cybersecurity by...

13 May 2024 3,792 0 View

For my msc project I want to build a cybersecurity framework to enhance organizational cybersecurity?

This cybersecurity framework is going to be tailored to SMEs in my country. Any suggestions please on how this framework can be evaluated as i don’t know how to go about achieving that at this...

17 November 2023 9,738 5 View

Msc Cybersecurity and Human Factors Project Topics Recommendations?

Please I need help. can someone please recommend Project topics i can choose from for my Msc in Cybersecurity and Human Factors degree. Thank you!

03 November 2023 7,880 5 View

Dear colleagues, there are two account of me: one "Norbert W. Brattig" , the other "Norbert Brattig". Please merge these bot under "Norbert Brattig"?

I hope all my publications can be find under the name "Norbert Brattig", since only in a number of oublications I used my name including my middle name. Thus, under "Norbert W. Brattig" the...

25 May 2023 6,499 1 View

In vitro lipid / cholesterol delivery to membrane preparations?

Is there an easy way to deliver lipids in vitro to crude membrane preparations? The membranes have been prepared from cell lysates by ultracentrifugation in non-denaturing, detergent-free buffer....

15 November 2022 8,241 0 View

Sucrose gradients and mass spectrometry to analyse protein complexes?

I was wondering if anyone could relate their experience with isolating and analysing protein complexes in complex samples (whole-cell lysates) using mass spectrometry on sucrose gradient...

16 March 2022 9,995 5 View

What solvent gradients can be used for lipid group fractionation by normal-phase hplc?

We a re trying to separate cholesterol, cholesterolesters, triglycerides and squalene by normal-phase hplc. We only need a crude group fractionation. However, with our solvent system consisting...

14 February 2022 6,507 2 View

Experience with artificial neural network software?

Has anyone experience with the software NeuralDesigner? Can anyone recommend this software oder recommend another software? I have to perform classification and prediction tasks with different...

10 November 2021 5,478 3 View

Is there an English Translation of the Carl Moller text: ZUR VERGLEICHENDEN ANATOMIE DER SILURIDEN?

I recently came across an anatomy text by Carl Moller that was published in 1915 but it is in German or Dutch neither of which I can understand. I would like to know if there is an English...

10 August 2024 4,347 1 View

Why Do TDS and EC Increase with Larger Wastewater Volumes, While BOD and COD Decrease?

I have carried out MFC experiments on three different volumes, 50, 500 and 1000 mL of wastewater. Results after MFC treatment shows that TDS and EC are more in larger volumes of water i.e. TDS and...

09 August 2024 9,621 0 View

Could it be a cell culture contamination?

Hello everyone! I observed in my culture (htert-RPE1 cells) an orange- red particle at the bottom of the dish. It is visible to the naked eye as a very very small red dot. Could it be a...

09 August 2024 2,824 3 View

How to confirm the site-directed mutagenesis result without performing NGS?

I'm cloning a fragment of 3200 nts into plasmid. The cloning was successful, however, 02 amino acids were mutated. Now I want to fix these 02 aa by site-directed mutagenesis technique using...

08 August 2024 4,645 2 View

Is there any way to quantify bacterial and fungal cells in their mixed culture?

I am working in fungal fermentation of soybean meal and there is bacterial growth in them at times. I am trying to quantify fungal cell counts and bacterial cells; but I haven't been able to do at...

07 August 2024 7,535 4 View

Hello all, Looking for international reviewer to review Ph.D thesis in wireless sensor network.Can anybody help?

My name is Apurva Saoji. I am a Ph.D scholar in Computer engineering in India. I am looking for international expert in reviewing my PhD thesis, "Competitive Optimization Techniques to Minimize...

07 August 2024 4,600 2 View

How to normalize and take the significance of the MTT OD values with 3 replicates for the same cell-line?

Hi, I have a question about normalizing the MTT OD values for doing the statistical analysis. So, if we have 3 different plates and we call them 3 different replicates, so, first we would...

07 August 2024 8,106 4 View

Why activated CAR-Jurkat cell could not kill targets?

Previously when I co-coluture anti-CD19(FMC63) CAR-Jurkat with Raji with E:T=5:1, Jurkat can eliminate Raji in 24h. However, when I test another CAR construct, although I can dectect totally CD69...

06 August 2024 641 2 View

How to isolate lymphocytes from mouse spleen?

I have tried several times to isolate lymphocytes from mouse spleen, but all attempts have been unsuccessful. I tried most available protocols. I used different dissociation media (HBSS with Ca...

04 August 2024 9,913 7 View

How to Add Missing Water Molecules in Protein-Membrane Simulations?

I have protein-membrane simulations (PDB, PSF, DCD) and have noticed that water molecules near the protein are not visible in the simulations. How can I fix this issue? Is there a way to place the...

04 August 2024 1,200 2 View