Norbert Duró

5 Questions 8 Answers 0 Followers

Questions related from Norbert Duró

Codon optimization of human gene for human host. Does it make sense?

Might be a silly question but is there any difference between plasmids translation efficiency carrying human genome sequence and codon optimized human sequence in human cell line?

17 June 2018 3,553 2 View

Which exon should I target for CRISPR-Cas9 system?

Is there any rule/preference which exon should I target to knock out the GOI by CRISPR-Cas9 system?

14 November 2016 4,957 5 View

Does my ligated plasmid ready for sequencing and is the purity OK?

I performed a ligation of 1.5 kb insert into a 4.2 kb vector and I got colonies on my kanamycin plate. I picked up 8 colonies and made a miniprep of them. 2 out of the 8 samples contained the...

16 February 2016 1,089 3 View

lost my pellet after washing with 70% EtOH and centrifuge during midiprep plasmid preparation.

During my plasmid purification with MN midiprep kit I loose my pellet after washing with 70% EtOH and centrifuge at 15,000 5min RT. I clearly see a huge amount of precipitate after using...

22 November 2015 3,967 3 View

How can I mount the fixed 293T cells cultured on coverslips to glass slides? Do I need to put any fluid between the coverslip and glass slide?

I would like to know how to mount the coverslips on glass slides? I culture 293T cells on coverslips for ICC. I want to fix them with 4% formaldehyde. After fixation, should I use any fluid as a...

23 July 2015 4,550 4 View