I am trying to reconstitute Cobalt protoporphyrin (from santa cruz) to use it to induce heme oxygenase 1 in endothelial cells and the DS mentions that I should use pyridine:H2O 1:1 but I dont know how to prepare it.
According to various research, soybean is not considered a pulse because of its high fat content.
30 June 2024 6,311 0 View
If so do you have to crosslink the compound to the oligo? If you have done this kind of experiment I would really appreciate any guidance/protocols. Thanks, Katie
21 May 2024 318 1 View
Hi all, Looking at the wikipedia page for photonic crystals, there is a section that reads: "One-dimensional photonic crystals can include layers of non-linear optical materials in which the...
02 February 2022 3,105 5 View
I am looking to use meta-ethnography and meta-analysis in qualitative health studies, but am doing quantitative studies as well, and am wondering if it would be useful for both.
13 June 2020 4,030 3 View
Greetings everyone, Would anyone be able to suggest or know of software to model the dosage of UV received by a surface from a source object? For example, I would like to model how a cylindrical...
15 May 2020 3,372 2 View
Greetings everyone, My lab, as with others many others, have shifted some of our efforts to aid in the global fight against COVID-19. As part of our efforts, we are looking to evaluate current...
06 May 2020 586 0 View
My question concerns a particular finding from a randomized, placebo-controlled within-group cross-over study. I have an interaction effect of relationship duration by treatment condition on...
04 March 2020 3,460 3 View
Cavity modes are a consequence of constructive interference of EM waves in a particular region. It is common knowledge that the (frequency) linewidth of a cavity resonance is broadened by both...
17 December 2019 2,866 6 View
I am planning to use radio telemetry in a study of grey-headed robins (Heteromyias cinereifrons). These birds weigh around 35 g and I have sourced glue-on transmitters weighing 0.9 g (
09 December 2019 3,934 3 View
The underlying physics behind quantization axes for the electrons spin has never really been clear to me. I saw the Pauli matrices in my quantum courses, but the definition of different principle...
04 August 2019 6,616 3 View
I have carried out MFC experiments on three different volumes, 50, 500 and 1000 mL of wastewater. Results after MFC treatment shows that TDS and EC are more in larger volumes of water i.e. TDS and...
09 August 2024 9,621 0 View
Hello everyone! I observed in my culture (htert-RPE1 cells) an orange- red particle at the bottom of the dish. It is visible to the naked eye as a very very small red dot. Could it be a...
09 August 2024 2,824 3 View
I am working in fungal fermentation of soybean meal and there is bacterial growth in them at times. I am trying to quantify fungal cell counts and bacterial cells; but I haven't been able to do at...
07 August 2024 7,535 4 View
Hi, I have a question about normalizing the MTT OD values for doing the statistical analysis. So, if we have 3 different plates and we call them 3 different replicates, so, first we would...
07 August 2024 8,106 4 View
Previously when I co-coluture anti-CD19(FMC63) CAR-Jurkat with Raji with E:T=5:1, Jurkat can eliminate Raji in 24h. However, when I test another CAR construct, although I can dectect totally CD69...
06 August 2024 641 2 View
i have sorted anti-NP specific plasma cells from bone marrow of C57BL/6 mice at certain times after immunization with variable counts and isolated total RNA using TRIZOL method for RT-PCR using...
05 August 2024 8,835 1 View
After immunohistochemistry of previously fixed in PFA and EtOH and then frozen 20 μm sections of zebrafish brain, DAPI staining is very weak (right) compared to the same sections stained without...
05 August 2024 9,637 2 View
Brain and body mass together are positively correlated with lifespan (Hofman 1993). The duration of neural development is one of the best predictors of brain size, and conception is the best...
05 August 2024 6,247 3 View
I have tried several times to isolate lymphocytes from mouse spleen, but all attempts have been unsuccessful. I tried most available protocols. I used different dissociation media (HBSS with Ca...
04 August 2024 9,913 7 View
I have protein-membrane simulations (PDB, PSF, DCD) and have noticed that water molecules near the protein are not visible in the simulations. How can I fix this issue? Is there a way to place the...
04 August 2024 1,200 2 View