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I am trying to reconstitute Cobalt protoporphyrin (from santa cruz) to use it to induce heme oxygenase 1 in endothelial cells and the DS mentions that I should use pyridine:H2O 1:1 but I dont know...
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I am reviving EAHY926 from -80 degrees at passage 38 and I centrifuge to get rid of the DMSO and yet the cells never attach so what could be the problem? BTW: I am using 10 % DMSO in DMEM while...
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