How to do the calculation for super oxide dimutase assay and how to calculate the R squared value in regression equation for catalase assay?

i have the enzymatic antioxidant assay by following the method of Misra and Fridovich, 1972 for super oxide dimutase and Luck, 1974 for catalase assay can any help me how to the calculation for...

11 February 2021 2,241 3 View

The peptide synthesized with CTC resin have mass more (+68 Da) than the desired mass of the synthesized peptide. What could be the reason?

I am synthesizing 7 amino acid long peptide sequence with CTC resin, 50oC coupling for 10 min. On cleavage (TFA/TIPS/Water 95:2.5:2.5) I got peptide with good yield. However, It has 68 Da more...

30 January 2021 8,483 3 View

How can we draw the graph of a function of complex variables involving two variables in Matlab?

Suppose the function is $f(z_1, z_2) = z_1^2+z_2^2$, where $z_1, z_2 \in \mathbb{C}$. How to implement the graph in Matlab in an given interval. Can anyone help?

11 January 2021 1,018 2 View

For VANET related issue /problems like congestion , MATLAB or NS2/NS3?? Which will be more suitable for implementation??

I have gone though few papers related to this problem. Researchers used NS2.35/2.38. and MATLAB. Also. My particular question is regarding congestion control . Which will be more suitable to use ?

22 December 2020 8,197 10 View

Physical significance of Gap between optical and acosutic branches in Phonon Dispersion?

Hello Everyone, Recently I am doing some Phonon branchesbased studies. For some materials the gap between the optical and acoustic branch is small, for some it's large and for some there is no...

04 November 2020 3,987 2 View

Can I perform islet isolation after storing the excised mouse pancreas for 3 months and what is the protocol for this process?

I have sacrificed treated BALB/C mice and collected the pancreas and stored in -20 degree centigrade for 3 months. Now I want to isolate the islets to study the release of different cytokines from...

08 October 2020 7,299 6 View

2 subsequent saccade and fixation duration after filtering 'unclassified' and 'eye not found' data in Eye tracking study?

My study is to use only fixation and saccade data. But after removing :unclassified' and 'eye not found' data. I come to know there is 2 back to back fixation duration or saccade what I need to...

28 September 2020 9,919 3 View

How informal learning can impact the learning culture of the organization?

Need to know specific parameters which impact the learning culture variables.

23 September 2020 233 4 View

How can i simulate dual-X second generation current conveyor using CMOS?

24 August 2020 8,067 4 View

In quantitative study, for calculating sample size, can the precision be taken 0.08?

In health related studies, can the level of precision be taken as 0.07 or 0.08 while calculating sample size?

19 July 2020 3,230 3 View

Can linear DNA be used as template for site-directed mutagenesis?

Is it possible to induce site-directed substitution mutation by quick-change method on linear dsDNA? or it has to be cloned in some vector? If yes, should it be treated with the Dpn1 enzyme...

03 March 2021 401 4 View

Does anyone have a recommendation for yeast reporter gene plasmid/protocol?

Hi, could anyone recommend a plasmid and/or protocol for reporter gene assay in S. cerevisiae? I want to assess the effect of growth conditions on a transcription factor, so I want to clone it`s...

01 March 2021 210 1 View

Expression cloning by using cDNA,do I need to modify the cDNA first?

I am going to have a expression cloning of mammalian gene by using shuttle plasmid to transforming the E.coli However I don't know I should only inserting the Coding sequence ,or I can...

28 February 2021 5,440 3 View

Difficulty in cloning of 1kb gene into vector?

I am facing difficulties in cloning a 1kb gene into a vector (pJIT163). I have my gene on interest (GOI) in pUC57 and want to clone in pJIT163 using SalI and BamHI restriction sites. I am getting...

25 February 2021 3,221 7 View

Why are Sanger Sequencing reads interrupted in some edited clones?

I have a Crispr/Cas9 edited bulk population and I can briefly check the indel ratios in the bulk population using Sanger sequencing. After cloning single cells, I wanted to check the indel types...

18 February 2021 8,198 6 View

Hi, can anybody help me with the suggestions how can I amplify a stress induced gene of 1107 bp?

I am trying to amplify the TGA1 (Transcription factor 1 ) of Arabidopsis ,which is 1107 bp. I have treated the plants with Salycylic solution over night and extracted the RNA and make cDNA. I...

17 February 2021 7,802 2 View

Alternatives to traditional cloning of insert into backbone (dsDNA)?

Hi, I am stuck with a cloning experiment: The insert & backbone(bb) share one compatible end at 3' end(NotI) and one incompatible end at 5' end (XbaI for insert; HindIII for backbone). So i...

16 February 2021 1,664 3 View

Why do I have trouble with cloning duplicate gene in the same plasmid?

Hello! I am trying to generate a construct in this format: seqA - seqB - linker - seqC - seqB into pSF-CMV-Ub-Puro-Ascl plasmid 0.5kb 2kb 66 0.25kb 2kb I have tried many...

15 February 2021 7,118 3 View

Help with Amyloid beta detection protocol (western-blotting)?

Hi everyone, I am a first year PhD student working on amyloidosis. I would like some tips and tricks to crack efficiently my current nightmare, i.e. the western blotting of the Amyloid beta and...

11 February 2021 2,513 3 View

Any probe or assay for the detection of Cysteine in yeast invivo ?

Dear fellow researchers, I would like to detect the cysteine levels for yeast in vivo. I would really appreciate if anyone could suggest me any fluorescent probe or assay kit available. Currently...

11 February 2021 3,292 3 View