Deepika Nag

2 Questions 3 Answers 0 Followers

Questions related from Deepika Nag

I am facing difficulty in cloning Kluyveromyces(yeast) B- gal gene(~3kb)into pET47 vector. Any suggestions?

Please suggest me if its possible to clone a 3kb yeast gene into a pET47b vector(~5.2kb) and further its expression into a E.coli expression host? In my case even ligation is not happening between...

29 October 2019 1,674 0 View

Cloning and expression of Yeast gene in bacterial host. Is it possible?

Can I clone and express a yeast gene of beta galactosidase into a bacterial host E. coli BL21? Will it effect the functionality of the protein?

19 August 2019 7,581 3 View