I want to clone a gene from Mycobacterium tuberculosis which codes for a secretory protein.I am expressing it in E.coli as a host. My query is should I design primers with the signal peptide or only for the mature protein.In most literature they have designed primers for the mature protein.Without signal peptide, what will be the effect on the expression of the protein.It is an extracellular protein.And If I include signal peptide,how will I get mature protein?

KIndly help