Risha Hazarika

15 Questions 11 Answers 0 Followers

Questions related from Risha Hazarika

Regarding resazurin assay?

I am doing a resazurin assay to determine the MIC of plant extracts against Mycobacterium tuberculosis. The problem is the MIC is coming at a very high value. I read in one paper that even if...

02 January 2024 8,004 2 View

How to detect a compound in hplc which has low wavelength?

I am trying to detect a plant compound Asiatic acid.In literature, the detection is very low 205 nm. When I try at this wavelength, there is huge noise and a baseline shift. I am using...

08 June 2022 9,456 0 View

How to measure mgGAE /g of dry plant i total phenolics content?

I am doing TPC assay. I obtained the equation y=0.0006x -0.0123 . I started with 8g of dry plant powder for extraction and dissolved it in 80 ml of methanol. I evaporated the total 80 ml volume...

22 November 2021 2,834 0 View

Extraction of aglycone flavonoids?

I want to extract quercetin from the leaves of Centella asiatica. It is an aglycone flavonoid and therefore is less polar compared to other flavonoids . What should be the appropriate solvent -...

15 October 2021 4,017 6 View

How to calculate total flavonoid content?

I have taken 1 gm of dry leaf powder ad extracted it in 10 ml of methanol solvent.I got crude extract of 70 mg.I did the AlCl3 assay for total flavonoid content for 2 mg / ml . I got the value of...

18 September 2021 701 2 View

Storage of dried and grounded plant material?

I have air dried and freeze dried my leaf sample .After grinding can I store it in a cool and dark place at room temperature until analysis....?

08 August 2021 1,067 3 View

Freeze drying of plant leaves?

I want to dry my plant sample (leaves) by freeze drying.Can someone give me a simple and detailed protocol on how to freeze dry in a lyophiliser . Like how to prepare the sample before freeze...

09 February 2021 2,508 5 View

Is this contamination in my lb agar plate?

I revived my e.coli dh5alpha glycerol stockin lb agar plate and I got single colonies but there is also dome extra white patches....what are these? ...Are these e.coli colonies ?

05 November 2020 5,385 6 View

Cloning and expression of a secretory protein?

I want to clone a gene from Mycobacterium tuberculosis which codes for a secretory protein.I am expressing it in E.coli as a host. My query is should I design primers with the signal peptide or...

21 October 2020 7,711 0 View

OD of E.coli culture?

The OD of the bacterial culture for Storing glycerol stocks is 0.8 and after how many hours does the OD reaches 0.8? Kindly help

16 September 2020 2,986 4 View

Prediction of amino acid sequence?

If I have a new gene sequence or nucleotide sequence obtained after gene sequencing and annotation, how can I further predict the amino acid sequence of any gene ?Kindly help

10 June 2020 4,937 4 View

Error in autodock?

I am experiencing this error in autodock 4.2.3 ,mgl tools 1.5.6 after setting the grid parameters and saving the gpf file because of which I am not able to view the results. >> Exception in...

18 March 2020 1,152 2 View

whta will happen if PCR Program is stopeed in between?

I accidentally set the initial denaturation at 95 degress for 1 min, but It should have been for 5 mins. I realised it after I started the program while it had reached denaturation step at 95...

28 August 2019 10,089 5 View

Has anyone tried the NID method of plasmid isolation?

I tried to isolate plasmid DNA by this method.I did not get any DNA.The link for the article is https://journals.plos.org/plosone/article?id=10.1371/journal.pone.0023457

22 August 2019 3,293 0 View

Why am I not getting DNA by alkaline lysis method?

I isolated plasmid DNA by alkaline lysis method.I am getting a very weird graph in nanodrop. No peak was seen at 260 nm.Can anyone please tell what is the problem?

19 August 2019 9,761 3 View