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Questions related from Risha Hazarika
I am doing a resazurin assay to determine the MIC of plant extracts against Mycobacterium tuberculosis. The problem is the MIC is coming at a very high value. I read in one paper that even if...
02 January 2024 7,892 2 View
I am trying to detect a plant compound Asiatic acid.In literature, the detection is very low 205 nm. When I try at this wavelength, there is huge noise and a baseline shift. I am using...
08 June 2022 9,353 0 View
I am doing TPC assay. I obtained the equation y=0.0006x -0.0123 . I started with 8g of dry plant powder for extraction and dissolved it in 80 ml of methanol. I evaporated the total 80 ml volume...
22 November 2021 2,724 0 View
I want to extract quercetin from the leaves of Centella asiatica. It is an aglycone flavonoid and therefore is less polar compared to other flavonoids . What should be the appropriate solvent -...
15 October 2021 3,947 6 View
I have taken 1 gm of dry leaf powder ad extracted it in 10 ml of methanol solvent.I got crude extract of 70 mg.I did the AlCl3 assay for total flavonoid content for 2 mg / ml . I got the value of...
18 September 2021 616 2 View
I have air dried and freeze dried my leaf sample .After grinding can I store it in a cool and dark place at room temperature until analysis....?
08 August 2021 997 3 View
I want to dry my plant sample (leaves) by freeze drying.Can someone give me a simple and detailed protocol on how to freeze dry in a lyophiliser . Like how to prepare the sample before freeze...
09 February 2021 2,434 5 View
I revived my e.coli dh5alpha glycerol stockin lb agar plate and I got single colonies but there is also dome extra white patches....what are these? ...Are these e.coli colonies ?
05 November 2020 5,301 6 View
The OD of the bacterial culture for Storing glycerol stocks is 0.8 and after how many hours does the OD reaches 0.8? Kindly help
16 September 2020 2,912 4 View
If I have a new gene sequence or nucleotide sequence obtained after gene sequencing and annotation, how can I further predict the amino acid sequence of any gene ?Kindly help
10 June 2020 4,878 4 View
I am experiencing this error in autodock 4.2.3 ,mgl tools 1.5.6 after setting the grid parameters and saving the gpf file because of which I am not able to view the results. >> Exception in...
18 March 2020 1,089 2 View
I accidentally set the initial denaturation at 95 degress for 1 min, but It should have been for 5 mins. I realised it after I started the program while it had reached denaturation step at 95...
28 August 2019 10,028 5 View
I isolated plasmid DNA by alkaline lysis method.I am getting a very weird graph in nanodrop. No peak was seen at 260 nm.Can anyone please tell what is the problem?
19 August 2019 9,684 3 View
