How axons look like when stained with osmium tetroxide?

Hi everyone, I am running experiments where I used osmium tetroxide to stain axons in the optic nerve and then image it with x ray microtomography. I don't know if my experiment worked as it seems...

02 March 2019 8,327 14 View

Anyone familiar with Amira image software?

Hello, is there anyone familiar with Amira 6.5? If so, I've been trying to calculate surface thickness of a segmented material but I think it does not work properly. Is there anyone familiar with...

10 November 2018 3,698 2 View

Tannic acid for x-ray microtomography: concentration?

Hi all, I will be doing some staining on a whole nerve and subject this to x ray microtomography. Has anyone ever used Tannic acid for this technique? If so, what's the concentration of the acid?...

05 June 2018 1,469 1 View

Amira and Image j: how can I reconstruct a 3D tissue?

Hi all, I've been starting using Amira for image analysis and I am having issues on aligning and subsequently merging 5 stacks. I need to have these 5 stacks completely aligned (these are from the...

04 May 2018 9,589 1 View

How to stain unmyelinated axons?

Hi, does anyone have any idea on how I can stain unmyelinated axons? I am looking for a method to stain unmyelinated axons within a whole tissue, meaning that I will not cut it in sections but...

02 March 2018 163 5 View

3D reconstruction in Amira?

Dear all, I am interested in reconstruct 3D images from Z stacks with Amira but I have never used it and I cannot find any specific tutorial for this. I explain. I acquired 3D stacks (coordinates...

11 December 2017 1,472 6 View

Quantify elastin within TPEF images + measruing collagen crimp on second harmonic generation images?

Hi everyone, 1.I am doing spectral imaging and linear unmixing on overlapped images and I wonder if possible to quantify the contribution of each component. I explain. I am trying to separate...

09 October 2017 3,105 2 View

Change in the shape of the spectrum, how to quantify?

Hello, I am currently working on assessing the elastin spectrum properties in different tissues and I'd like to compare the elastin spectral signature between tissues to see whether or not there...

06 July 2017 10,001 8 View

Quantify Second harmonic and two photons signals?

03 April 2017 1,280 1 View

Are there any sticky microscope spacers without leaking the liquid?

Hello, I am working with optic nerve sections and I am trying to image them using a new method which does not involve mounting the sample on a slide and cover it with a coverslip. I am placing the...

08 September 2016 3,021 6 View

How to compare two groups with only two measurements?

Hiiiii everyone! I have an enquiry on statistical analysis. I was looking for many forum and it's still cannot solve my problem. I want to compare means of two groups of data but only with two...

03 March 2021 8,796 3 View

Binary Logistic Generalized Linear Mixed model in SPSS. Is it doable?

I want to analyses the proportion of swimming sperm of three species of fish in two salinities. To analyse the proportion of swimming sperm in a Generalized Linear Model, I would use a Binary...

03 March 2021 2,297 3 View

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10?

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10? Since, the growth curve from day 2 to 10 won't be like the "Logistic curve" it might not follow logistic...

03 March 2021 1,401 3 View

What test should i use in comparing the correlation between categorical and continuous variables?

Dear all, I have the variable (Surgery duration) represented by values in minutes and the variable (preoperative neuropraxia cases) represented as (Yes/No) values. I want to compare these two...

03 March 2021 5,427 5 View

Logistical regression using SPSS for a dichotomous dependent variable, with an independent variable of 1, 2, 5 and 10 seconds?

I have an experiment with 28 participants The independent variable is the times 1, 2, 5, and 10 used as the cross hairs during a task These 4 times were tested using 4 different blocks, each...

03 March 2021 9,692 2 View

How to find the mean, SD and CI in SPSS?

Hi, I hope that someone can help me with this task: I have 5 groups of participants which are numbered in SPSS from 1 to 5 (according to their group number) and are all under the variable group....

03 March 2021 506 3 View

What degree of missing data in a particular case/participant results in Expectation-Maximisation no longer working?

I'm dealing with a mediation model and am using the PROCESS module in SPSS. Due to SPSS and PROCESS being limited in the imputation methods - being unable to handle multiple imputation - the other...

02 March 2021 4,362 1 View

How to code open-ended questions in SPSS?

Hello all, In SPSS I am going to code 2 open-ended questions. I have already read all the answers and I made a list of the most important categories to which I can code the answers. This question...

02 March 2021 1,757 4 View

The procedure compute?

02 March 2021 5,204 3 View

Does anyone know what a '.' means in SPSS?

Hi everyone! I was wondering if anyone would be so kind as to help me with regards to my data set on SPSS! I was checking for multivariate outliers via the standardised residuals ('Linear...

02 March 2021 7,910 3 View