How axons look like when stained with osmium tetroxide?

Hi everyone, I am running experiments where I used osmium tetroxide to stain axons in the optic nerve and then image it with x ray microtomography. I don't know if my experiment worked as it seems...

02 March 2019 8,327 14 View

Tannic acid for x-ray microtomography: concentration?

Hi all, I will be doing some staining on a whole nerve and subject this to x ray microtomography. Has anyone ever used Tannic acid for this technique? If so, what's the concentration of the acid?...

05 June 2018 1,469 1 View

Circular statistic: SPSS?

Hi All, I have some angle measurements in degrees and I am not sure how to do circular stat. I know Oriana software but it is not for free download. I don't think SPSS does it. What about...

04 May 2018 8,106 3 View

Amira and Image j: how can I reconstruct a 3D tissue?

Hi all, I've been starting using Amira for image analysis and I am having issues on aligning and subsequently merging 5 stacks. I need to have these 5 stacks completely aligned (these are from the...

04 May 2018 9,589 1 View

How to stain unmyelinated axons?

Hi, does anyone have any idea on how I can stain unmyelinated axons? I am looking for a method to stain unmyelinated axons within a whole tissue, meaning that I will not cut it in sections but...

02 March 2018 163 5 View

3D reconstruction in Amira?

Dear all, I am interested in reconstruct 3D images from Z stacks with Amira but I have never used it and I cannot find any specific tutorial for this. I explain. I acquired 3D stacks (coordinates...

11 December 2017 1,472 6 View

Quantify elastin within TPEF images + measruing collagen crimp on second harmonic generation images?

Hi everyone, 1.I am doing spectral imaging and linear unmixing on overlapped images and I wonder if possible to quantify the contribution of each component. I explain. I am trying to separate...

09 October 2017 3,105 2 View

Change in the shape of the spectrum, how to quantify?

Hello, I am currently working on assessing the elastin spectrum properties in different tissues and I'd like to compare the elastin spectral signature between tissues to see whether or not there...

06 July 2017 10,001 8 View

Quantify Second harmonic and two photons signals?

03 April 2017 1,280 1 View

Are there any sticky microscope spacers without leaking the liquid?

Hello, I am working with optic nerve sections and I am trying to image them using a new method which does not involve mounting the sample on a slide and cover it with a coverslip. I am placing the...

08 September 2016 3,021 6 View

What are the values needed for gabor filter's parameter for cifar10 data?

Hi, I implemented a code to gabor filter cifar10 data but the images after being filtered and stacked are not clear like the original images. I think the problem is in the way I am using the...

03 March 2021 6,317 1 View

New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before?

Question to you and THEM, the New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before? There appears to be a core problem for...

02 March 2021 3,024 2 View

Is it necessary to report effect size (ES) in statistics of biomedical research given that we already report p values routinely?

Pls share your thoughts

01 March 2021 7,330 4 View

Which reagent is best among 'Dimethylglyoxime" and "Dithiothreitol" for spectroscopic determination of Nickel ?

What I think is Dithiothreitol. As it is water-soluble and does not form a precipitate. But, the thing is it contains sulphur which is quite hazardous to aquatic animals.

01 March 2021 8,374 4 View

How to identify what type of fillers are in a polymer?

Hello everyone, I have some PHAs blended with fillers and nucleating agents, yet I don't know what kind of fillers or nucleating agents. The producer has shut down and there's no way to get in...

24 February 2021 1,356 8 View

Is it mandatory to specify Subjects Combinations in Generalized Linear Mixed Models (SPSS)?

Hi all, I have data with two levels: lets think of the classic example of students and schools. When running the analysis in SPSS and selecting the Random Effects, we can select (we can or we...

24 February 2021 7,379 4 View

Do you think there is cognitive oversaturation in the current pandemic? What impact can this have? And other manifestations?

Dear colleagues, Do you think there is cognitive oversaturation in the current pandemic? What impact can this have? And other manifestations? The question is mainly aimed at adolescents (pupils,...

23 February 2021 7,796 4 View

Is it possible that Cancer cells could survive in heparinized blood for 48 hours?

Hi everyone, I am just wondering if we incubated 200 microliter of whole blood with 50 microliter of cancer cells(1bout 5000cells) do you think that cancer would survive for 48 hours?

23 February 2021 3,840 3 View

How to minimise the risk of being abused the profile of potential participants tagged in a social media post?

Dear colleagues, I got this one in one project I did ethics review. It's great to hear about your thoughts/experiences. "Doing an open recruitment online (i.e., via Facebook) requires public...

22 February 2021 3,322 3 View

What type of SPSS analyses should I use for my Dissertation?

I'm currently working on my Dissertation and I'm trying to think of the best analyses that I should use for my study. I'm using three questionnaires for this and two different groups, 1st group...

22 February 2021 1,351 4 View