11 Questions 15 Answers 0 Followers
Questions related from Laura Paletto
Hi everyone, I am running experiments where I used osmium tetroxide to stain axons in the optic nerve and then image it with x ray microtomography. I don't know if my experiment worked as it seems...
03 March 2019 8,403 14 View
Hello, is there anyone familiar with Amira 6.5? If so, I've been trying to calculate surface thickness of a segmented material but I think it does not work properly. Is there anyone familiar with...
11 November 2018 3,792 2 View
Hi all, I will be doing some staining on a whole nerve and subject this to x ray microtomography. Has anyone ever used Tannic acid for this technique? If so, what's the concentration of the acid?...
06 June 2018 1,554 1 View
Hi all, I've been starting using Amira for image analysis and I am having issues on aligning and subsequently merging 5 stacks. I need to have these 5 stacks completely aligned (these are from the...
05 May 2018 9,655 1 View
Hi All, I have some angle measurements in degrees and I am not sure how to do circular stat. I know Oriana software but it is not for free download. I don't think SPSS does it. What about...
05 May 2018 8,342 3 View
Hi, does anyone have any idea on how I can stain unmyelinated axons? I am looking for a method to stain unmyelinated axons within a whole tissue, meaning that I will not cut it in sections but...
03 March 2018 223 5 View
Dear all, I am interested in reconstruct 3D images from Z stacks with Amira but I have never used it and I cannot find any specific tutorial for this. I explain. I acquired 3D stacks (coordinates...
12 December 2017 1,623 6 View
Hi everyone, 1.I am doing spectral imaging and linear unmixing on overlapped images and I wonder if possible to quantify the contribution of each component. I explain. I am trying to separate...
10 October 2017 3,206 2 View
Hello, I am currently working on assessing the elastin spectrum properties in different tissues and I'd like to compare the elastin spectral signature between tissues to see whether or not there...
07 July 2017 10,125 8 View
Hello, I'll need to quantify, somehow, the SHG (second harmonic generation) signal within collagen fibres and TPEF (two-photons excited fluorescence) signal within elastic fibres. Does anyone...
04 April 2017 1,349 1 View
Hello, I am working with optic nerve sections and I am trying to image them using a new method which does not involve mounting the sample on a slide and cover it with a coverslip. I am placing the...
09 September 2016 3,109 6 View