Is it possible to get intense signal using Cy3 filters for FAM-labeled probes?

My colleague and I did FISH using a FAM-labeled probe. We observed signals using FITC filters. However, when we tried to use the Cy3 filter, we could see intense and distinct signals not observed...

03 April 2017 8,759 3 View

How can I view the GC% of my DNA sequence in a graphical view?

Like I want to make a 100bp or 120bp sliding window GC% graph. Thank you!

08 September 2015 6,191 0 View

How can I compute sequence similarities using MEGA?

I have more than 10 sequences. I want to know the sequence similarity or identity of one sequence against each other sequences. How can I do that? Thank you.

11 December 2014 5,159 2 View

What online database should I use to BLAST my list of ~5-15 bp DNA sequences?

Hello. I have several short-length DNA sequences. I want to know which online database you recommend to know if my sequences match to known DNA patterns/motifs? Thanks a lot.

11 December 2014 8,186 4 View

How do I find my gene's UTR regions?

I found a gene in my plant sample through a homology search. I only got the CDS region though from our RNA database. How can I know the extent of the boundaries of the 5' and 3' regions of this...

08 September 2014 9,440 5 View

Why do I get the cloned insert size rather than the plasmid size when I gel analyze after maxiprep?

I did maxiprep plasmid/BAC isolation. When I did gel analyze, I was expecting for the classical two bands for the supercoiled and linear plasmids. Instead, I got real intense bands that...

06 July 2014 8,001 13 View

How can I estimate the GC content of a genome?

Is there any method that I can use on genomic DNA to estimate the GC content of a particular plant species' genome? I was wondering whether High Resolution Melt (HRM) could do it.

03 April 2014 2,996 12 View

Is there any enzyme that preferentially degrades double-stranded DNA and keeps single-stranded ones?

I want to isolate single-stranded DNA from a mixture with double-stranded ones in a reassociation experiment. Any help will be greatly appreciated. Thank you!

31 December 2013 4,987 3 View

Is there any laboratory that gives workshop of Fluorescence activated Chromosome Sorting (FACS)?

I was asked by my professor to isolate a single chromosome from my plant sample. I learned this could be done by flow karyotyping and then sorting using FACS. The thing is I don't know anyone in...

01 February 2013 8,759 3 View

What primer sequences are used to amplify telomeres of plants?

I want to amplify about 1 kb sequence of my plant sample. Is it possible using the repetitive telomere sequence?

11 December 2012 8,828 5 View

MD MOE RMSD

How to calculate the RMSD values for a MD simulation using MOE?

07 August 2021 0 0 View

MD MOE RMSD

How to calculate the RMSD values for a MD simulation using MOE?

07 August 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence?

I am on the lookout for the Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence. Does anyone know where I can find it? Thank you in advance

03 March 2021 3,568 1 View

What kind of fluid could I use for a pitfall trap that also does not invalidate molecular testing?

Hi, I want to start testing pitfall trap to obtain ants samples, but I need to conduct molecular analysis on those insects. So, what kind of fluid can I use? Ethanol expires too early and I need...

03 March 2021 5,978 5 View

How to increase a model accuracy ?

dear community, my model is based feature extraction from non stationary signals using discrete Wavelet Transform and then using statistical features then machine learning classifiers in order to...

03 March 2021 6,994 5 View

No title

02 March 2021 3,060 3 View