Please is there any reason that will make synthesized iron nano particle to be brown in color instead of black?

Hello all, Please I was synthesizing Iron nanoparticle using Eucalyptus leaf extract which after the first trial that I got black color particles. However, subsequently I am getting brown color...

13 December 2020 2,205 6 View

Which method is the most suitable for determination of LD50 of a plant extract?

As we have different methods for the determination of LD50 of a plant extract, such as OECD (up and down method), Lorke method, probit method, etc., so, which one among them is most suitable that...

25 September 2020 4,460 2 View

What happen if I run native protein under denaturing PAGE? Will the size larger than expected?

Hai. I break the cells containing my expressed protein (r-msAdh1) using non - denaturing lysis buffer. Then I run 10 uL lysate under denaturing polyarylamide gel (SDS-PAGE). But I observed that my...

12 March 2020 2,288 9 View

How can I calculate/determine protein purity after running SDS-PAGE and staining with CBB?

I am writing a report and I am supposed to determine the purity of the protein sample? Samples were ran on SDS-PAGE and stained with CBB. Using the Rf value and plotting a standard curve, I was...

02 January 2019 179 3 View

Machine learning or Evolutionary Algorithm?

I have one question regarding the Machine learning (ML) /Evolutionary Algorithm(EA) method. One of my research objectives is to develop new equation taking as a starting point the pool of...

16 October 2017 8,730 4 View

Which reference gene is suitable for normalizing miRNA measurement in serum samples?

Which reference gene is suitable for normalizing miRNA measurement in serum samples?

07 October 2017 2,277 3 View

What is the procedure of using High Performance Liquid Chromatography analysis machine?

I want to analyse concentration of lovastatin in the fermented rice straw extract.

01 June 2016 8,269 3 View

What is the relevance of the mass attenuation coefficient to the radiation detector response?

what is the relevance of the mass attenuation coefficient to the radiation detector response?

29 February 2016 533 5 View

Is there a validated questionnaire tool translated into Bahasa Malaysia which assess aberrant opioid medication related behaviour?

For example, questionnaire tools such as SOAPP-R, ORBIT scale, or PODS. Thank you.

24 January 2016 234 1 View

No title

31 December 2014 1,266 3 View

Chlorinated organic formation in odor control scrubbers using hypochlorite?

I'm involved in a study of odor control technologies for municipal wastewater treatment plant. One of the control options involves a chemical 2-stage (acid/alkaline) packed bed scrubber. The...

03 March 2021 3,661 2 View

What solvent can I used for Paal Knorr reaction between 6-amino hexanoic acid and 2.5 hexanedione?

I am try to make the Paal Knorr reaction between 2.5 hexnedione and 6-amino hexanoic acid, my problem is type of solvent to use, because 6-hexaminohexanoic acid is soluble in water but I am not...

02 March 2021 4,443 2 View

Does Napabucasin soluble in dichloromethane and in ethanol?

I need to prepare a solution of Napabucasin (inhibitor of cancer cell stemness) in dichloromethane. The drug is expensive, so maybe somebody already tried to do it. If it is soluble, what is the...

02 March 2021 9,945 3 View

Ester cleavage conditions?

Hi all, I need to cleave the ester in Ethyl 2-amino-1H-imidazole-5-carboxylate to make the carboxylic acid and retrieve the product from solution to use in downstream applications of linking to...

01 March 2021 8,766 3 View

Can chitosan alone can act as a reducing agent for Cu(0) nanoparticle sythesis?

[Synthesizing Cu nanoparticles without using chemical reductants]

01 March 2021 8,359 1 View

What is the state of the drug inside solid lipid nanoparticles (SLNs)?

I am a little confuse with the mechanism of solid lipid nanoparticles (SLNs). The main point of encapsulating drugs into SLNs is because some drugs have low solubility in water. Therefore, SLNs...

01 March 2021 3,703 3 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

How to remove DCU and HOBT from my reaction?

I am doing a acid and a chiral amino alcohol reaction using DCC and HOBT in DCM or THF? Can anyone suggest me how to remove DCU and HOBT from a reaction mixture??

28 February 2021 2,153 1 View

What is the most common number of epitopes in an multi-epitope Vaccine?

hi everyone, I'm interested in the field of vaccine design specifically multi-epitope cancer vaccines , I want to know if there is a minimum and maximum number of epitopes we could use in a...

27 February 2021 3,882 3 View

I want to performe MALDI-TOF MS for bacterial identification, I would appreciate it if anyone can tell me the matrix solution preparation protocol ?

based on what I've studied on multiple papers, for peptide and protein identification Alpha-cyano-4-hydroxycinnamic acid (CHCA) is an appropriate matrix to use, I also know that the CHCA powder...

26 February 2021 6,799 3 View