Which method is the most suitable for determination of LD50 of a plant extract?

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M. A. Azadi

Probit method.

Jürg Gertsch

I agree with the Probit method for blotting. But I would ask the question how it is administered in vivo (to an animal). Most of the natural products present in an extract are not systemically bioavailable to a degree that makes them bioactive as they are efficiently metabolized or not even absorbed (polyphenols, tannins, etc). I would never persue tox studies with i.p administration as this is highly artificial.