Why is my protein lysate not separated on a SDS gel?

Hello. I prepared protein lysates from Hek293t cells by using RIPA buffer. When I run my samples on a SDS gel, they don't separate. I have attached pictures of the gel after running it and of a...

01 August 2019 4,852 4 View

Is it okay to digitally magnify images of tissue stained via immunohistochemistry?

We took images at 10X of tissue from the central nucleus (CeA) of the amygdala, but those images included tissue outside of the CeA. In order to simplify things and keep from having to re-image...

10 May 2018 9,982 4 View

Fast image segmentation methods?

What is the difference in computational time between watershed segmentation and color threshold (in one color plane)? My images usually have one to three objects that needs to be separated from a...

30 May 2016 9,356 4 View

What is the most precise method for cell counting?

It seems that the method of cell counting with a TC20 cell counter by Bio-Rad is associated with with high variations. Therefore, I am searching for a better method to improve the accuracy. Can...

19 October 2014 3,735 7 View

Is crowd funding a viable alternative now that research grant awards are at an all-time low?

Crowd funding has been successfully used to launch many new inventions, musical and artistic projects. Will scientists now turn to crowd funding or is this merely a passing fad?

06 November 2012 2,809 13 View

After a reference from Int J Body Compos Res, can any body help?

Hi, I am after the reference below, my library says it cannot obtain a copy either locally or internationally, any help appreciated! Chris Wang ZM, Heshka S, Wielopolski L, Pi-Sunyer FX, Pierson...

03 March 2021 6,193 1 View

Chlorinated organic formation in odor control scrubbers using hypochlorite?

I'm involved in a study of odor control technologies for municipal wastewater treatment plant. One of the control options involves a chemical 2-stage (acid/alkaline) packed bed scrubber. The...

03 March 2021 3,661 2 View

What solvent can I used for Paal Knorr reaction between 6-amino hexanoic acid and 2.5 hexanedione?

I am try to make the Paal Knorr reaction between 2.5 hexnedione and 6-amino hexanoic acid, my problem is type of solvent to use, because 6-hexaminohexanoic acid is soluble in water but I am not...

02 March 2021 4,443 2 View

SDS page software for analysis?

I did sds page to determine the difference in protein expression between 3 types of vaccine , but i don't have scanner or densitometer available. . so i wonder if there is a software to analyze...

02 March 2021 6,270 3 View

Which of the Covid-19 Vaccines would elicit the best immunoprotection and why?

I want to know which of the available Covid-19 Vaccines would elicit the best immunological response and why

02 March 2021 1,388 3 View

Is the sinovac vaccine safe and effective for people over the age of 60 ?

Dear respective researchers, I was asked by my institution to be vaccinated this week using the sinovac vaccine. However, from some of the information I got, this vaccine is not recommended for...

01 March 2021 7,781 6 View

Ester cleavage conditions?

Hi all, I need to cleave the ester in Ethyl 2-amino-1H-imidazole-5-carboxylate to make the carboxylic acid and retrieve the product from solution to use in downstream applications of linking to...

01 March 2021 8,766 3 View

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01 March 2021 3,905 5 View

How vaccines of covid 19 work?

Millions ask how vaccines of covid 19 be successful and safe, its here to explain generally how vaccine of pfizer work ,mRNA vaccines teach our cells how to make a protein that will trigger an...

28 February 2021 2,723 4 View

How to remove DCU and HOBT from my reaction?

I am doing a acid and a chiral amino alcohol reaction using DCC and HOBT in DCM or THF? Can anyone suggest me how to remove DCU and HOBT from a reaction mixture??

28 February 2021 2,153 1 View