Can you recommend a method to monitor cell viability in vitro, while an embryo is developing in culture. Thanks!
You can detect the viability of embryos by montor the the further development in in vitro tissue culture or staining with defrential staining for counting the cell number or by embryo transfer .
You can test the viability using Triphenyltetrazolium chloride (TTC test). Check out the procedure for your tissue
Probably the best way is direct observation of emryonic cleavage and growth. One can see significant increase in cell number in embryos every 24 hours.
Hi, please check this article
Journal of Assisted Reproduction and Genetics, Vol. 15, No. 8, 1998 pages 455-458
"Human Embryo Viability: What Determines Developmental Potential, and Can It Be Assessed?"
http://www.springerlink.com/content/vl3262068r291141/
Good luck!
Neha, and this TTC test allow me to use embryos then for other test or application?
TAHNKS ALL FOR YOUR ANSWERS!
I think the best way is direct observation of emryonic cleavage and growth. One can see significant increase in cell number in embryos
Hi ,
May be this article can help you. If you have problems to download I have the article , you can give your email and I´ll sent you.
Regards
http://onlinelibrary.wiley.com/doi/10.1111/j.1439-0264.2011.01133.x/abstract
You could analyse the media your embryos have been cultured in . Look for the work on pyruvate, glucose from david gardmer and Henri Leese ore older papers from Renard and Yves Ménézo. Good luck
to evaluate a viability you can use TUNEL to detect apoptotic cells or if you have to maintain you embryo in culture, so you can analyse by electrophoresis the media of culture every day from J1 until J5 (secretomic and metabolic)
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