9 Questions 23 Answers 0 Followers
Questions related from Jesica Canizo
I have a doubt of compatibility between the cap strip form Thermo Scientific and Bio-Rad plates for real time PCR. I know that Opticon thermocycler read fluorescence from the top of the plates, I...
10 October 2014 8,273 4 View
Does anyone know about which carrier is better for RNA extraction: LPA (linear acrilamide), tRNA or glycogen? I need to analyze samples with low quantities of RNA, make c-DNA and Real time PCR. I...
08 August 2014 1,055 9 View
Does anybody know if I can use the termocycler for real time PCR that doesn't have the adequate instrument to measure the ROX fluorophore with the master mix that have it? I thought that I will...
07 July 2014 6,587 2 View
I dont have signal in my real time PCR with two genes, one a reference gene b-actin and the other a pluripotency gene.
03 March 2013 8,199 21 View
I am designing my real time PCR pair of primers. I put them into oligoanalyzer to determine hairpin loops, homo and heterodimer formation. What are the limts for a good design regarding these 3...
02 February 2013 4,936 14 View
Why the two options? (a basic question, but I always buy flat cap, for evaporation issues, and I don't understand why dome cap exists).
11 November 2012 5,499 14 View
I have a problem thinking on a paper discussion. When sb extracts RNA from a pool of embryos of different stages to analyze pattern expression of one gene: the number of cells impact in the...
05 May 2012 3,621 4 View
Has anyone a good tip to do nice mounted sample for immunocytochemistry? I have tried to have good images, but, sometimes, my embryos are close to the boundary of the mounting reagent and I have...
03 March 2012 8,470 15 View
Can you recommend a method to monitor cell viability in vitro, while an embryo is developing in culture. Thanks!
03 March 2012 5,451 9 View
