I would like to test cells for pathogens prior to injecting them into mice. I am aware of diagnostic laboratories offering this service, but they are quite expensive. Are there any commercially available kits to test for a range of pathogens? Thank you!
Dear Isabel,
Companies will rip you off, do it yourself. It very much depends what pathogens you would like to detect in what kind of cells. Usually, the most sensitive and robust are PCR-based kits. For example, I had very good experience with this kit from Sigma: MP0035 for detecting Mycoplasm in ES cells. You may also find PCR assay-based protocols in the net. Then, you just need to order specific primers and standard PCR reagents, to detect pathogen's DNA. But the kits are already optimized, might save you some time (=money).
Cheers,
Lech
- Badges
- Science topic
- Similar topics
- Biological Science
- Molecular Cell Biology
- Culture Cells
- Cell Line