Hi, could you expand on your definition of extended culturing please? I tend to prefer to use up my primary cells within a week of acquisition, but the paper below gives a guide on more long term cell culture and cell line prep. Hope this helps!

http://www.ncbi.nlm.nih.gov/pmc/articles/PMC348863/

Identification and distribution of developing innate lymphoid cells in the fetal mouse intestine

Jennifer K Bando,1,

Hong-Erh Liang1, 2,

& Richard M Locksley1, 2,

Journal name:

Nature Immunology

Volume:

16,

Pages:

153–160

Year published:

(2015)

Thanks!