Is it better to use U-bottomed (round-bottomed) or V-bottomed 96-well plates for FACS staining? BD for example suggests U-bottomed in their protocols, but I was told by many researchers that U-bottomed plates should instead be used for culturing cells and V-bottomed for staining, because the narrow bottom of V-bottomed plate will allow better binding of antibodies to the antigens, as cells and antibodies are more 'snug'.
Thank you!
I don't think this will matter. You can use any one of them as long as you are comfortable. You can perform flow staining in a glass tube, eppendorf as well as polypropylene flow tube.
Thanks Asif. A lot of researchers I spoke with are very strongly against round-bottomed plates for FACS staining so I was wondering if this is a general opinion.
I always culture and stain in the same plate - the U-bottomed plate. Someone told me you might lose cells in extensive staining protocols, but I perform live/dead, cell surface, fixation, and ICS and still have well enough cells to acquire in FACS. Sadly, the cell culture plates does not work with the BD plate reader, so a last transfer to V-bottomed plates is still required.
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