Lech Kaczmarczyk

4 Questions 30 Answers 0 Followers

Questions related from Lech Kaczmarczyk

What is the function of sucrose in lysis buffer for isolation of intact nuclei?

What is the function of sucrose in lysis buffers for isolation of intact nuclei? I observed that nuclei pellets resuspend easier in buffers without sucrose, and have a higher tendency to clump...

06 June 2016 4,017 10 View

Is it possible to use Miltenyi MACS micro Beads and/or uMACS beads in tubes instead of columns?

Hi All, I was just wondering if anyone tried to use any of the Miltenyi beads in tubes instead of columns, with a standard magnets compatible with the "tube" magnetic separation format. My guess...

05 May 2016 5,594 3 View

What is the best strategy for FACS staining of nuclear protein for sorting fixed nuclei?

Hi All, I need to FACS-sort formaldehyde-fixed nuclei from mouse brain tissue. What is the best strategy for staining of intranuclear protein for immunolabeling for FACS (staining of soluble...

05 May 2016 6,505 3 View

Any advice on QIAzol vs. RLT buffer for purifying small RNAs on Qiagen columns?

I use Qiagen RNeasy Micro kit for small RNA purification. My starting material are eukaryotic cells, or material previously lysed in a different lysis buffer. Therefore, cell disruption is not a...

10 October 2015 5,420 3 View