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Questions related from Isabel Holmes
Hi, is anyone aware of papers showing evidence of extended culturing and/or in vitro manipulation of primary mouse ILCs? If so, could you please attach the references? Thank you!
05 May 2016 3,197 3 View
I'm analysing my cells in FlowJo now after having acquired them. I see that '96% of events are on the chart edges'. Does that mean that I have set my FSC and SSC voltages incorrectly? I acquired...
04 April 2016 2,926 8 View
Is it better to use U-bottomed (round-bottomed) or V-bottomed 96-well plates for FACS staining? BD for example suggests U-bottomed in their protocols, but I was told by many researchers that...
04 April 2016 5,844 3 View
I would like to test cells for pathogens prior to injecting them into mice. I am aware of diagnostic laboratories offering this service, but they are quite expensive. Are there any commercially...
03 March 2016 7,137 1 View
What's the 'best practice' when it comes to thawing frozen cells? What I usually is do thaw them one afternoon and split them the next day. Should I instead leave them in culture for longer before...
03 March 2016 538 3 View
