How to avoid ulcers in s.c. injected tumors ?

Recently, we have injected s.c. B16F10 tumor cells in BL/6 mice. At around 12-15th day some animals showed ulcerations in tumors , with a big hole in the middle (it looks like a volcano). We have...

01 March 2021 9,938 3 View

What is the standard protocol in doing replicates for C. elegans lifespan assay on solid media (thru worm picking)?

I would like to know what is the standard protocol in the number of worms per plate and number of plates per concentration of extract X in a lifespan assay done in solid media and not automated...

23 February 2021 9,933 1 View

Should the AUC in a concentration-time graph of a subcutaneous implant be roughly the same for poeple?

I have 2 concentration time curves of a subcut drug implant. the first group has a peak around the 50 day mark where as the second group does not. Both groups received the same implant by the same...

10 February 2021 2,305 3 View

SPSS AMOS: Running my structural model decreases the CFI significantly - trying to account for it results in an unidentified model. What can I do?

Hi, after a thorough EFA and CFA, I've created my structural model (with imputed factor scores) in AMOS and ran it. While it provided me with all path coefficients, I found that the CFI had...

06 January 2021 5,087 4 View

Does anyone know how to fix the "A sample of parameter values was inadmissible"-error in SPSS AMOS when imputing factors?

Hi all, I'm trying to impute factors after testing my model for CMV and therefore included a common latent factor. Unfortunately, I always get the same error: "A sample of parameter values was...

02 January 2021 6,647 5 View

How can I measure the bacterial growth in a wet fabric in a petri dish?

I have to measure the growth of the bacteria in a piece of fabric for my senior design project. I don't know how to measure it. The only way that I know is by the naked eye or by the microscope....

23 November 2020 2,897 6 View

Calculating effect sizes without knowing the sample size by subgroups?

Hello! I am calculating effect sizes for a research synthesis of the literature (e.g. Hedges' g), but many of the original publications do not provide the sample size (N) by subgroups. I see...

13 September 2020 7,587 3 View

How to download a spatial subset of the new worldclim 2.1 layers using the R console?

Hello I have tried to download the new version of the worldclim bioclimatic layers in the R console, since this way I can download spatial subsets. Does anyone have a code that works with the new...

10 September 2020 1,948 5 View

Does an oral & Maxillofacial Medicine Specialist or physician have experience prescribing buspirone to a patient with SSRIs-related bruxism?

Does an oral & Maxillofacial Medicine specialist or physician have experience prescribing buspirone to a patient with SSRIs-related bruxism?

09 September 2020 5,952 4 View

Who can help me justifying intervals in my analysis?

Dear researchers, In my study, I have qualitatively (with a bit of quantitative) found results when comparing how cities and states attract international organizations. These results I coin low,...

18 August 2020 9,608 3 View

Can we classify HV and LV lines based on line length?

I have dataset which shows the length of power lines. I need to classify the lines based on the line length. Is there a rule to classify the High voltage (HV) and low voltage (LV) lines based on...

03 March 2021 4,116 4 View

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02 March 2021 3,060 3 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

Do you need the CMV enhancer before the CMV promotor for a proper function of CMV on the gene that follows?

I'm a student and I have to produce a cell line with a knockin for the NRF2 gene with GFP. I have to put a promotor in front of the GFP because the gene will be too far away from the promotor of...

28 February 2021 7,127 2 View

What could be some reasons why unstimulated monocytes are getting activated?

Im doing PBMC isolation -> CD14+ enrichment using magnetic beads -> stimulation setup. My negative control is just cells in cRPMI but they seem to get activated over and over again.

28 February 2021 7,883 3 View

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28 February 2021 9,936 2 View

Has anyone had luck using cell lines other than HEK 293 (such as COS1) to package lentivirus?

I am looking to package lentivirus in endothelial cells but I am opposed to using 293 cells due to personal ethics regarding the source. I have done some reading and saw that COS1, COS7, & CHO...

25 February 2021 3,030 1 View