Rosa Gallo

4 Questions 8 Answers 0 Followers

Questions related from Rosa Gallo

How does fixation and permeabilization can affect the number of cells in flow cytometry?

Hi all, I carried out a flow cytometry in adipose tissue with a new permeabilization and fixation method in order to improve the intracellular staining for TFs... However, comparing with the...

17 August 2022 901 0 View

Cell line expressing Rorcg T ?

Hi, Is there a cell line Rorc gamma T positive that I can use to perform assays in vitro? Thanks! Rosa

08 March 2022 3,092 1 View

Is M-CSFR 1 a specific markers to identify the total population of macrophages?

Hi all! I want to carry out immunofluorescence to detect macrophages. Is M-CSFR 1 specific to identify macrophages? I am a little bit confused because I have read that this receptor is also...

06 February 2022 8,128 2 View

How to analyze flow cytometry data?

Hello, I carried out a multicolour immunophenotyping flow cytometry and now I am analyzing the data got. Basically, it's not clear to me how to analyze the data got from flowJo in order to get...

10 July 2021 8,755 3 View