it is being two months at different times and different situations, i face these [ short video attached] specific contaminants. the cells i got them from different individuals. i have checked all culture materials and medias at different instances: like incubating media alone, filtering medias, changing gloves, wearing long protection gloves, sterilized tips...etc. all come out negative. in addition the incubator and laminar flow hood is used by others no such instances, at least for those whom I ask. more clarification can be provided upon request. so i am curious what is the main source of these contaminants and in which material might be.
any sort of solution is of paramount importance.
Hi Rezene Habte
I would suggest discarding the current cell line and media. Prepare a fresh DMEM media with antibiotics: Penstrep (penicillin and streptomycin) and ciprofloxacin.
While passaging the cells, try washing the cells multiple times with autoclaved 1X PBS.
To check if your media or PBS has any contamination, put a test plate (only Media or Media + PBS) in the incubator for 24 to 48 H and check for contamination. While preparing media, you can keep the required autoclaved materials like (filter, bottles, Water, etc.) in UV for surface sterilization.
I hope this helps.
Best,
Harsh
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