Cattle mesenchymal stem cell

More Riris Lindiawati Puspitasari's questions See All

How to measure pH of high concentration salt solution (17-19% NaCl solution)?

How to measure pH of high concentration salt solution (17-19% NaCl solution)? Do we need a special pH meter specially designed for pH measurement in high concentration salt solutions? If yes,...

07 April 2021 8,987 4 View

What is recommended and opened real-time or nearly real time geospatial data used in flood risk assessment and regional planning on a detailed scale?

I asked recommended real-time or nearly real-time data source for any parameters for flood risk on a microscale analysis for urban area cases in developing countries that had agglomeration urban...

18 February 2021 2,969 2 View

How to analysis orientation molecule adsorbed on mineral clay molecular dynamics using gromacs?

I'm still confused. Whether to determine the methylene blue orientation, I have to use the order parameter (gmx order) or the gmx angle? I want to determine the orientation of the methylene blue...

31 December 2020 2,554 3 View

Solvent for Dimehypo (Thiosultap disodium; thiosultap sodium; bisultap)?

Does anyone know what the best solvent for dimehypo (thiosultap disodium, thiosultap sodium; bisultap)? Can this compound dissolve in organic solvents (acetonitril, acetone, ethanol, etc)? i...

16 April 2020 4,054 3 View

Can someone give a reason why my gc-ms results in larger molecular weight compounds come out first than compounds with smaller molecular weight?

can anyone give a reason if the gc-ms data I obtained for the C11 chain (octyl acrylate) came out first and had a retention time of 4.074, the second compound that came out was octane having a...

10 December 2019 3,333 7 View

What is the best method to determine NaOCl in green soybean?

I have used NaOCl in various concentrations for the green soybean sterilization process. I need to know the residual concentration of NaOCl remaining in soybeans because NaOCl should not be in...

25 August 2019 8,842 2 View

Mental health and public health

what mental health and the correlation to public health? What a differences mental health in public health, mental health in phsycology, and mental health in medical?

01 January 1970 8,279 14 View

What are the emerging trends and opportunities for STEM education and careers in the online space?

10 August 2024 507 0 View

Why Do TDS and EC Increase with Larger Wastewater Volumes, While BOD and COD Decrease?

I have carried out MFC experiments on three different volumes, 50, 500 and 1000 mL of wastewater. Results after MFC treatment shows that TDS and EC are more in larger volumes of water i.e. TDS and...

09 August 2024 9,621 0 View

Could it be a cell culture contamination?

Hello everyone! I observed in my culture (htert-RPE1 cells) an orange- red particle at the bottom of the dish. It is visible to the naked eye as a very very small red dot. Could it be a...

09 August 2024 2,824 3 View

Is there any way to quantify bacterial and fungal cells in their mixed culture?

I am working in fungal fermentation of soybean meal and there is bacterial growth in them at times. I am trying to quantify fungal cell counts and bacterial cells; but I haven't been able to do at...

07 August 2024 7,535 4 View

How to normalize and take the significance of the MTT OD values with 3 replicates for the same cell-line?

Hi, I have a question about normalizing the MTT OD values for doing the statistical analysis. So, if we have 3 different plates and we call them 3 different replicates, so, first we would...

07 August 2024 8,106 4 View

Why activated CAR-Jurkat cell could not kill targets?

Previously when I co-coluture anti-CD19(FMC63) CAR-Jurkat with Raji with E:T=5:1, Jurkat can eliminate Raji in 24h. However, when I test another CAR construct, although I can dectect totally CD69...

06 August 2024 641 2 View

How much total RNA concentration to be extracted from sorted plasma cells from bone marrow of C57BL/6 mice for RT-PCR ?

i have sorted anti-NP specific plasma cells from bone marrow of C57BL/6 mice at certain times after immunization with variable counts and isolated total RNA using TRIZOL method for RT-PCR using...

05 August 2024 8,835 1 View

How to isolate lymphocytes from mouse spleen?

I have tried several times to isolate lymphocytes from mouse spleen, but all attempts have been unsuccessful. I tried most available protocols. I used different dissociation media (HBSS with Ca...

04 August 2024 9,913 7 View

How to Add Missing Water Molecules in Protein-Membrane Simulations?

I have protein-membrane simulations (PDB, PSF, DCD) and have noticed that water molecules near the protein are not visible in the simulations. How can I fix this issue? Is there a way to place the...

04 August 2024 1,200 2 View

How to use Density Functional Theory to calculate carrier mobilities of solid system?

Hello, everyone. I have tried to determine carrier motilities of some materials, by Density Functional Theory, using Quantum ESPRESSO. There are a few methods to do it, like a package called...

04 August 2024 8,894 1 View

Saurabh Mandal

hi Riris Lindiawati Puspitasari

Check this latest review paper, which nicely described MSCs isolation from bovine tissue. you may find your answer from Ref. 71 to 82.

Article An Update on Applications of Cattle Mesenchymal Stromal Cells

Also, check this: Article Post-calving umbilical cord tissue offcut: A potential sourc...

We do human UC MSCs isolation, we tried enzymatic digestion as well as tried without any enzyme. In bovine UC, I can see many just scraps the cord or WJ portion of the tissue in the media so that cells will ooze out in the plate.

Hope you find it helpful.

Regards

Saurabh

Riris Lindiawati Puspitasari

Hello Mr Saurabh Mandal

I will check them

Thank you a lot Saurabh Mandal