Hi, can anybody help me with the suggestions how can I amplify a stress induced gene of 1107 bp?

I am trying to amplify the TGA1 (Transcription factor 1 ) of Arabidopsis ,which is 1107 bp. I have treated the plants with Salycylic solution over night and extracted the RNA and make cDNA. I...

17 February 2021 7,802 2 View

How can we make remote teaching and learning more interesting and engaging?

We ( Higher Education) started with Zoom, then Google Meet, and this term we will be using more updated and smarter system MS TEAM to make online teaching more participatory and engaging and...

12 August 2020 9,154 42 View

How to fix atoms with same coordinate error?

I am getting a fatal error in gromacs while creating a GRO file and ligand topology file under the amber force field. The acype of python providing the error message. Parameter was: Charge =...

29 July 2020 3,347 4 View

Correlation coefficient and Mutual information range?

what is the difference between correlation coefficient(CC) and mutual information(MI)? what is the range of both methods (CC and MI)?? I want to evaluate image registration through these two methods.

16 July 2020 2,767 4 View

Is there any way to implement the MATLAB code in the genetic algorithm which solves optimization problem of image registration?

I want to design an algorithm which solves the optimization problem in image registration through genetic algorithm and also design stationary field. Give me your suggestion for this idea is it...

14 April 2020 3,237 4 View

How to improved results of medical image registration ?

I have improved diffeomorphic log demons image registration. Now what should i do for improving results. what should i add in my registration algorithm. Can you suggest me something who are expert...

29 March 2020 2,054 3 View

How can I solve error, getting "dplyr" package for R 3.6.0 ?

15 January 2020 3,150 3 View

How to get uniform coating of graphene oxide?

Hello all, Could anyone suggest me how to get a very uniform graphene oxide film? I used dip coating, spray coating and spin coating of GO on glass slide. But none of these methods worked. When I...

23 July 2017 6,469 5 View

How to determine the latitude and longitude of a target?

I have the position of a RADAR station and some aitrcrafts' azimuth, elevation and range data. How can I calculate the latitude longitude of those aircrafts?

30 August 2016 6,636 6 View

How to find an optimum route, in a network, with minimum travel time and maximum served demand by usin Genetic Algorithm in MatLab?

I have network comprising of nodes and links, also the travel time information and demand at certain nodes ; all in the form of matrix. If i know the Origin and destination point, how can i find...

15 April 2016 5,400 2 View

Does Napabucasin soluble in dichloromethane and in ethanol?

I need to prepare a solution of Napabucasin (inhibitor of cancer cell stemness) in dichloromethane. The drug is expensive, so maybe somebody already tried to do it. If it is soluble, what is the...

02 March 2021 9,945 3 View

Could you please recommend some good journal that accept very long revie papers (approximately 6000+ words) in neurosurgery, cancer biology ?

Could you please recommend some good journal that accept very long revie papers (approximately 6000+ words) in neurosurgery, cancer biology ?

01 March 2021 8,087 3 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

Is it possible that Cancer cells could survive in heparinized blood for 48 hours?

Hi everyone, I am just wondering if we incubated 200 microliter of whole blood with 50 microliter of cancer cells(1bout 5000cells) do you think that cancer would survive for 48 hours?

23 February 2021 3,840 3 View

The PC12 cell line characteristics?

Hello dear scientists, Does the undifferentiated PC12 cell line in terms of chromosome characteristics, proliferation, and drug reactions have the characteristics of cancer cells? Where is it...

21 February 2021 8,945 3 View

What is the minimum number of exosomes/ml to have a good yield of total RNA for Illumina sequencing?

I am using IZON qEV2 columns to isolate exosomes from 100 ml of cancer cell conditioned medium. in NTA analysis I usually see concentration of 2x10^9 particles/ml. But when I proceed with this...

20 February 2021 6,879 3 View

Can the viability assays (ex: MTT/CCK-8) measure cell proliferation? or are they solely viability assays?

I want to conduct a proliferation assay to test a chemotherapy combination on a cancer cell line. At first, I thought about the MTT assay. But now, all sources are saying that these do not measure...

14 February 2021 3,142 7 View

How much should I centrifuge cells in agarose-coated plates to get spheroids?

Right now I am using HT29 cells, but I will also use HCT116 and probably breast cancer cell lines.

09 February 2021 2,063 6 View

C-4 I cervical cancer cell line tissue culture protocol?

Hi there! My lab has been brought an array of C-4 I cell line. The cells did not succeed in attaching to the bottom of the flask and remained floating in the media. Although we followed the...

04 February 2021 10,018 5 View