Dear Tabassum,
In this article (attached), you can note that researchers conserved their antioxidant enzymes in −20 °C to minimize their activities, and prevent their degradation process.
Best wishes
Temperature for storing antioxidant samples, can affect their activities, across the time. We store our at -80 C.
it depends on the time you will put before use. but for a better guarantee store at -80°C and you can keep there for at least 6 months
Dear Tabassum, I agree with Kevin. In our laboratory, we try to finish measurements within 6 months, however, there is a lot of data suggesting that enzymes will not change significantly up to one year if they are stored properly and at -80 C.
store it in a brown bottle to avoid light oxidation
store it in a refrigerant or at low temperature
Effect of storage temperature on enzyme activity and antioxidant capacity have been discussed in this article which deals with plant. you may have a look: http://www.scielo.org.mx/scielo.php?script=sci_arttext&pid=S1027-152X2015000300002
Different storage methods such as Freeze-drying, from at 20°c & freezing -80°c extract store from fresh sampled material
Activity of several ROS(reactive oxygen species) freezing samples are shock frozen in liquid nitrogen and stored -20°c or -80°c for later use.
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