How can we make remote teaching and learning more interesting and engaging?

We ( Higher Education) started with Zoom, then Google Meet, and this term we will be using more updated and smarter system MS TEAM to make online teaching more participatory and engaging and...

12 August 2020 9,154 42 View

How to fix atoms with same coordinate error?

I am getting a fatal error in gromacs while creating a GRO file and ligand topology file under the amber force field. The acype of python providing the error message. Parameter was: Charge =...

29 July 2020 3,347 4 View

Correlation coefficient and Mutual information range?

what is the difference between correlation coefficient(CC) and mutual information(MI)? what is the range of both methods (CC and MI)?? I want to evaluate image registration through these two methods.

16 July 2020 2,767 4 View

Is there any way to implement the MATLAB code in the genetic algorithm which solves optimization problem of image registration?

I want to design an algorithm which solves the optimization problem in image registration through genetic algorithm and also design stationary field. Give me your suggestion for this idea is it...

14 April 2020 3,237 4 View

How to improved results of medical image registration ?

I have improved diffeomorphic log demons image registration. Now what should i do for improving results. what should i add in my registration algorithm. Can you suggest me something who are expert...

29 March 2020 2,054 3 View

How can I solve error, getting "dplyr" package for R 3.6.0 ?

15 January 2020 3,150 3 View

How to get uniform coating of graphene oxide?

Hello all, Could anyone suggest me how to get a very uniform graphene oxide film? I used dip coating, spray coating and spin coating of GO on glass slide. But none of these methods worked. When I...

23 July 2017 6,469 5 View

How to determine the latitude and longitude of a target?

I have the position of a RADAR station and some aitrcrafts' azimuth, elevation and range data. How can I calculate the latitude longitude of those aircrafts?

30 August 2016 6,636 6 View

How to find an optimum route, in a network, with minimum travel time and maximum served demand by usin Genetic Algorithm in MatLab?

I have network comprising of nodes and links, also the travel time information and demand at certain nodes ; all in the form of matrix. If i know the Origin and destination point, how can i find...

15 April 2016 5,400 2 View

For amniotic fluid culture, can versene be used to detach the attachments from the flask?

18 April 2015 7,372 2 View

Can linear DNA be used as template for site-directed mutagenesis?

Is it possible to induce site-directed substitution mutation by quick-change method on linear dsDNA? or it has to be cloned in some vector? If yes, should it be treated with the Dpn1 enzyme...

03 March 2021 401 4 View

In PRIMER, is it possible to downweight certain taxa in multivariate community datasets to reflect uncertainty around identification??

I have a dataset with about 80 different species. As usual, some species are very easy to identify with certainty whereas others are more difficult, which means that I am less certain of my...

03 March 2021 8,066 4 View

PACYC PCR not working after several attempts. Would anyone have any suggestions?

So, I have been trying to run a pACYC PCR which will be used later on for a Gibson Assembly. However the PCR is not working. I have already tried gradient PCR and changing extension time; however...

02 March 2021 1,146 2 View

Does anyone have a recommendation for yeast reporter gene plasmid/protocol?

Hi, could anyone recommend a plasmid and/or protocol for reporter gene assay in S. cerevisiae? I want to assess the effect of growth conditions on a transcription factor, so I want to clone it`s...

01 March 2021 210 1 View

How to calculate the annealing temperature of a primer pair?

I have to amplify a gene and my primers just reached. The Tm for Forward primer is 64.2, and that of reverse primer is 65.5. Can some one suggest how to get the best annealing temperature? Thanks...

01 March 2021 360 7 View

Expression cloning by using cDNA,do I need to modify the cDNA first?

I am going to have a expression cloning of mammalian gene by using shuttle plasmid to transforming the E.coli However I don't know I should only inserting the Coding sequence ,or I can...

28 February 2021 5,440 3 View

Anybody with an idea on how do design specific primers for detecting tomato resistance genes tm-1, TM-2 and Tm2^2?

I am trying to identify these 3 genes among some tomato cultivar collections and after aligning some sequences from NCBI, I couldn't find unique sequences to target for specific primers. There...

28 February 2021 606 3 View

What is the ideal efficiency for a qPCR?

hello everyone, I need to do standard curves for my qPCR, what is the ideal efficiency range? I tried a primer (Mglu2 receptor) that gave an efficiency of 90.2%. Is it accepted?

28 February 2021 1,254 3 View

MiRNA qpcr problem?

Dear All, mirna primer showing some problem in the melting curve? any idea why? As attached is the melting curve. The forward sequence is obtained from miRBase and reverse primer is universal.

28 February 2021 5,008 4 View

Calculating volume of DNA required from DNA concentration?

Hi I am a bit confused. They are asking me to find out the volume of DNA required in ul (a total of 30-100 ng for genomic DNA) from the DNA concentration in the nanodrop reading which was 404.8...

26 February 2021 5,029 2 View